Balcaitis 2017 Thesis

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Balcaitis D (2017) The investigation of derivates of the 1,4-naphthoquinone effects to mitochondrial oxygen consumption in glioblastoma cells. Diploma Thesis p43.

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Balcaitis D (2017) Diploma Thesis

Abstract: The aim of research – to investigate and compare the effects of different concentrations of 1,4-naphthoquinones (lawsone, plumbagin, menadione, juglone) on rat glioblastoma cell culture and assess their mitochondrial oxygen consumption.

Methods: C6 cell culture was used for experiments. Cell breeding, preparation, density calculation was executed by the methods of cell cultivation. Mitochondrial respiration rate was registered using oxygraphic system Oxygraph-2k.

Goals of the study: 1. To investigate and compare the effect of different concentrations of lawsone on C6 cell culture and assess mitochondrial respiration rate. 2. To investigate and compare the effect of different concentrations of plumbagin on C6 cell culture and assess mitochondrial respiration rate. 3. To investigate and compare the effect of different concentrations of menadione on C6 cell culture and assess mitochondrial respiration rate. 4. To investigate and compare the effect of different concentrations of juglone on C6 cell culture and assess mitochondrial respiration rate.

Results: Our results have shown that 0,5-5,5 µM concentrations of juglone increases VL. Higher concentrations of juglone separates oxidation and phosphorylation in mitochondria. Lawsone, used in 10-195 µM concentrations, increases both VL and VADP, but does not fully separate oxidation and phosphorylation. Results have shown that concentrations between 0,5 to 3,5 µM of plumbagin increases VL by 119-223 %. 1 µM concentration of plumbagin, used in ox-phos capacity, fully separates the processes in mitochondria. Concentrations of menadione (0,5-5,5 µM) increases VL by 31-150 %. Higher concentrations fully separates oxidation and phosphorylation. 2 µM of menadione also separates the processes when used in ox-phos capacity.

Conclusions: 1,4-naphthoquinones (lawsone, plumbagin, menadione, juglone) have different approach to mitochondrial respiration rate and it all depends on concentrations. The weakest effect has lawsone because it does not have big effect on VL and ox-phos capacity. The biggest effect is made by plumbagin.

Keywords: 1, 4-napthoquinones, Juglone, Lawsone, Menadione, Plumbagin, C6 cell culture Bioblast editor: Kandolf G O2k-Network Lab: LT Kaunas Briedis V


Labels: MiParea: Respiration, Pharmacology;toxicology  Pathology: Cancer 

Organism: Rat  Tissue;cell: Nervous system, Other cell lines 


Coupling state: OXPHOS  Pathway: N, NS  HRR: Oxygraph-2k 

Labels, 2018-02