MiPNet22.03 IOC119 Innsbruck AT

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MiPNet22.03 IOC119 Innsbruck AT

Publications in the MiPMap
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Innsbruck AT, 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic IOC119.

»

OROBOROS (2017-02-27) Mitochondr Physiol Network

Abstract:
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MitoFit Coaching Days - Basic. Innsbruck, Austria; 2017 Feb 28-Mar 03.


O2k-Network Lab: AT_Innsbruck_OROBOROS, AT_Innsbruck_MitoFit


Programme

Tuesday 28th Topic Room
17:00 - 18:00 Welcome - common aims MiPArt
Wednesday 1st Topic Room
09:00 Mitochondrial and cell research MiPArt
10:00 OroboPOS service and O2k instrumental setup - overview with videoclips MiPArt
10:30 Hands-on: OroboPOS service and O2k instrumental setup MiPArt
12:00 Lunch break
13:00 Instrumental quality control 1: oxygen calibration MiPArt
14:00 Instrumental quality control 2: Instrumental O2 background MiPArt
15:00 Coffee
15:30 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. MitoFit ORO Lab
14:30-18:00 Hands-on: Instrumental quality control 1 and 2. MitoFit ORO Lab - MiPArt
Thursday 2nd Topic Room
09:00 Demo: Instrumental quality control 1. Introduction to SUIT protocols RP1&RP2 MiPArt
10:00 Coffee
10:30 O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. MiPArt
12:00 Lunch break
13:00 Introduction to DatLab analysis MiPArt
15:00 Coffee
15:30-18:00 Individual DatLab analysis + summary MiPArt
Friday 3rd Topic Room
09:00 SUIT protocols MiPArt
10:30 Coffee
11:00 Q&A session on HRR and OXPHOS analysis: Design of experimental protocols MiPArt
12:00 Lunch break
13:00 O2k-Core and O2k-Multisensor MiPArt
14:00 DatLab analysis MiPArt
15:00 Coffee
15:30 DatLab analysis MiPArt
16:15 The Bioblast wiki, O2k-Network and MITOEAGLE - feedback & conclusions MiPArt
17:30 Farewell activity MiPArt

General information

The MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the O2k-Core, instrumental assembly and technical service, the DatLab software, oxygen sensor calibration, oxygen flux analysis of respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
» Information on travel and venue: IOC Innsbruck.

Registration

Fully booked.
Maximum number of 6 participants.

Tutors



Participants

Participant Institution
Anja Bufe AT Innsbruck OROBOROS: OROBOROS INSTRUMENTS, Innsbruck (AT)
Eugenia Carvalho US AR Little Rock Boersheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
Matthew Cotter US AR Little Rock Boersheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
Ivone Leong SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE)
Yue Hugh Guan
Yue Hugh Guan CN Shanghai Yi X: East China University of Science and Technology, Shanghai (CN)
Vasco Sequeira Oliveira NL Amsterdam Wuest RC: VU University Medical Center, Amsterdam (NL)
Anna-Maria Wintler SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE)
Evelyn Zacharewicz NL Maastricht Schrauwen P: Department of Human Biology and Movement Sciences, Maastricht University (NL)

Contact

instruments@oroboros.at


OROBOROS INSTRUMENTS
high-resolution respirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
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IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.


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Additional: ORO, IOC, 2017, MitoFit, Next