Mufazalov 2009 Mol Biol (Mosk)

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Mufazalov IA, Pen'kov DN, Cherniak BV, Pletiushkina OIu, Vysokikh MIu, Kirpichnikov MP, Dolgikh DA, Kruglov AA, Kuprash DV, Skulachev VP, Nedospasov SA (2009) Preparation and characterization of mouse embryonic fibroblasts with K72W mutation in somatic cytochrome c gene. Mol Biol (Mosk) 43:648-56.

» PMID: 19807028

Mufazalov IA, Penkov DN, Cherniak BV, Pletiushkina OIu, Vyssokikh MY, Kirpichnikov MP, Dolgikh DA, Kruglov AA, Kuprash DV, Skulachev VP, Nedospasov SA (2009) Mol Biol (Mosk)

Abstract: Mouse embryonic fibroblasts (MEF) with point mutation in somatic cytochrome c gene were generated and characterized. It was shown that substitution of lysine for tryptophan in position 72 (K72W) decreased the proapoptotic functions of cytochrome c in response to staurosporin treatment without disrupting its respiratory functions. The presence of this mutation did not affect the pattern of cytochrome c gene expression or its localization inside the cell. These cell cultures therefore represent an interesting model for study apoptotic signaling and physiological functions of cytochrome c.

Keywords: [Article in Russian]; Staurosporin, Cytochrome c gene

O2k-Network Lab: RU Moscow Skulachev VP


Labels: MiParea: Respiration  Pathology: Cancer 

Organism: Mouse  Tissue;cell: Fibroblast 


Regulation: Cyt c 


HRR: Oxygraph-2k