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O2k-Catalogue» OROBOROS O2k-Catalogue, Order @OROBOROS

Description O2k-Fluorometer (OROBOROS O2k-Fluorometer, OROBOROS Oxygraph-2k Fluorometer) - the experimental system complete for high-resolution respirometry (HRR) combined with fluorometry. The O2k-Fluorometer includes the O2k-Core, O2k-Fluo LED2-Module and TIP2k, and supports all other add-on O2k-Modules of the Oxygraph-2k.

The O2k is a sole source apparatus with no other instruments meeting its specifications.

Product ID 10010-01
Type O2k, O2k-Module, MultiSensor, O2k-Fluorescence LED2-Module, Catalogue
Link MiPNet17.05 O2k-Fluo LED2-Module

MitoPedia methods: Respirometry, Fluorometry 

MitoPedia O2k and high-resolution respirometry: O2k hardware 

The O2k-Fluorometer[1] does not include, but fully supports additional add-on MultiSensor Modules of the Oxygraph-2k, such as the O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module.

  1. A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see here.

The O2k-Fluorometer consists of

Title Description Product id Product image
O2k-Core OROBOROS O2k-Core - the experimental system complete for basic high-resolution respirometry (HRR). The O2k-Core includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System and the O2k-Titration Set. The O2k-Core supports all add-on O2k-Modules of the O2k, and is a component of the O2k-Fluorometer.

On-line display of oxygen flux (rate of respiration) is provided in addition to the conventional 'oxygraphic' plot of oxygen concentration over time. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Peltier temperature control provides a thermal stability at ±0.002 °C in the range of 4 °C to 47 °C at typical constant room temperature. Electronically controlled PVDF or PEEK stirrers are integrated in the two-chamber design of the O2k, and a barometric pressure transducer enables automatic oxygen calibrations implemented in the DatLab software.

The O2k is a sole source apparatus with no other instruments meeting its specifications.
TIP2k-Module TIP2k-Module - Titration-Injection microPump (TIP2k) for two-channel operation with the O2k-Core with automatic control by DatLab of programmable titration regimes and feedback control (oxystat, pH-stat). The TIP2k-Module includes TIP2k-Accessories: 4 Microinjection syringes, 1 Pkg. TIP2k-Needle Spacers with mounting tool, 1 TIP2k-Needle Safety Support and 1 Pkg. TIP2k Filter Papers. 11100-02
TIP2k with 200 mm3 microsyringe.JPG
O2k-Fluo LED2-Module The O2k-Fluo LED2-Module is a component of the O2k-Fluorometer. It is an amperometric add-on module to the O2k-Core (Series D upwards), adding a new dimension to HRR. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex red), ATP production (Magnesium green), mt-membrane potential (Safranin, TMRM), Ca2+ (Calcium green), and numerous other applications open for O2k-user innovation. The O2k-Fluo LED2-Module consists of optical sensors for both O2k-Chambers (LEDs for green and blue excitation), optical filters, Fluorescence-Control Unit for regulation of light intensity, data input into the O2k-Main Unit, and the updated DatLab software. 12100-01
Fluorescence-Control Unit lettered.jpg

O2k-Fluorometry Workshops

» OROBOROS O2k-Workshops

O2k-Fluorometer Manual: O2k-Fluo LED2-Module

» O2k-Manual: O2k-Fluo LED2-Module

See also

 »O2k-Demo experiments
 »MitoPedia: Fluorometry


O2k-Publications in the MiPMap

»O2k-Publications: Topics

Sort in ascending/descending order by a click on one of the small symbols in squares below.
Default sorting: chronological. Empty fields appear first in ascending order. 
Was published in year Has title Tissue and cell Cell line Mammal and model Nonmammal
Bouitbir 2016 Antioxid Redox Signal 2016 Bouitbir J, Singh F, Charles AL, Schlagowski AI, Bonifacio A, Echaniz-Laguna A, Geny B, Krähenbühl S, Zoll J (2016) Statins trigger mitochondrial ROS-induced apoptosis in glycolytic skeletal muscle. Antioxid Redox Signal 24:84-98. Skeletal muscle Other cell lines Rat
Karlsson 2016 Shock 2016 Karlsson M, Hara N, Morata S, Sjövall F, Kilbaugh T, Hansson MJ, Uchino H, Elmér E (2016) Diverse and tissue-specific mitochondrial respiratory response in a mouse model of sepsis-induced multiple organ failure. Shock 45:404-10. Nervous system
Nemeth 2016 FASEB J 2016 Németh B, Doczi J, Csete D, Kacso G, Ravasz D, Adams D, Kiss G, Nagy AM, Horvath G, Tretter L, Mócsai A, Csépányi-Kömi R, Iordanov I, Adam-Vizi V, Chinopoulos C (2016) Abolition of mitochondrial substrate-level phosphorylation by itaconic acid produced by LPS-induced Irg1 expression in cells of murine macrophage lineage. FASEB J 30:286-300. Liver Mouse
Du 2016 J Exp Biol 2016 Du SN, Mahalingam S, Borowiec BG, Scott GR (2016) Mitochondrial physiology and reactive oxygen species production are altered by hypoxia acclimation in killifish (Fundulus heteroclitus). J Exp Biol 219:1130-8. Liver Fishes
Kacso 2016 Biochem J 2016 Kacso G, Ravasz D, Doczi J, Németh B, Madgar O, Saada A, Ilin P, Miller C, Ostergaard E, Iordanov I, Adams D, Vargedo Z, Araki M, Araki K, Nakahara M, Ito H, Gál A, Molnár MJ, Nagy Z, Patocs A, Adam-Vizi V, Chinopoulos C (2016) Two transgenic mouse models for β-subunit components of succinate-CoA ligase yielding pleiotropic metabolic alterations. Biochem J 473:3463-85. Heart
Nervous system
Liepinsh 2016 Biochem J 2016 Liepinsh E, Makrecka-Kuka M, Volska K, Kuka J, Makarova E, Antone U, Sevostjanovs E, Vilskersts R, Strods A, Tars K, Dambrova M (2016) Long-chain acylcarnitines determine ischaemia/reperfusion-induced damage in heart mitochondria. Biochem J 473:1191-202. Heart Rat
MiPNet18.10 O2k versus multiwell 2015-10-05
O2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis.
Fibroblast Human
MiPNet18.05 Amplex-Mouse-heart 2015-06-16
O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate.
Heart Mouse
MiPNet20.13 Safranin mt-membranepotential 2015-06-15
O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin.
Nervous system Mouse
MiPNet20.14 AmplexRed H2O2-production 2015-06-15
O2k-Fluorometry: HRR and simultaneous determination of H2O2 production with Amplex Red.
Heart Mouse
MiPNet17.05 O2k-Fluo LED2-Module 2015-06-15
O2k-Fluo LED2-Module.
MiPNet18.06 Amplex-Yeast 2015-06-15
O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast.
Saccharomyces cerevisiae Fungi
O2k versus multiwell respirometer 2015-05-21
O2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis.
Fibroblast Human
Du 2015 J Biol Chem 2015 Du J, Rountree A, Cleghorn WM, Contreras L, Lindsay KJ, Sadilek M, Gu H, Djukovic D, Raftery D, Satrustegui J, Kanow M, Chan L, Tsang SH, Sweet IR, Hurley JB (2015) Phototransduction influences metabolic flux and nucleotide metabolism in mouse retina. J Biol Chem 291:4698-710. Nervous system
Islet cell;pancreas;thymus
Krumschnabel 2015 Methods Mol Biol 2015
O2k-Protocols contents
Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61.
Nervous system Mouse
Makrecka-Kuka 2015 Biomolecules 2015
O2k-Protocols contents
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38.
Nervous system
HEK Human
Kluckova 2015 Cell Death Dis 2015 Kluckova K, Sticha M, Cerny J, Mracek T, Dong L, Drahota Z, Gottlieb E, Neuzil J, Rohlena J (2015) Ubiquinone-binding site mutagenesis reveals the role of mitochondrial complex II in cell death initiation. Cell Death Dis 6:e1749. Lung;gill Fibroblast
Other cell lines
Other mammals
Roy Chowdhury 2015 Biosci Rep 2015 Roy Chowdhury SK, Djordjevic J, Albensi B, Fernyhough P (2015) Simultaneous evaluation of substrate-dependent oxygen consumption rates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep 36. Nervous system Mouse
Iftikar 2014 Thesis University of Auckland 2014 Iftikar FI (2014) Testing the role of heart mitochondrial stability and function in heart failure of ectotherms exposed to heat stress. Thesis University of Auckland:167pp. Heart Fishes
Elustondo 2014 Biochim Biophys Acta 2014 Elustondo PA, Negoda A, Kane CL, Kane DA, Pavlov EV (2014) Spermine selectively inhibits high-conductance, but not low-conductance calcium-induced permeability transition pore. Biochim Biophys Acta 1847:231–40. Liver Rat
Soltysinska 2014 PLoS One 2014 Soltysinska E, Bentzen BH, Barthmes M, Hattel H, Thrush AB, Harper ME, Qvortrup K, Larsen FJ, Schiffer TA, Losa-Reyna J, Straubinger J, Kniess A, Thomsen MB, Brüggemann A, Fenske S, Biel M, Ruth P, Wahl-Schott C, Boushel RC, Olesen SP, Lukowski R (2014) KCNMA1 encoded cardiac BK channels afford protection against ischemia-reperfusion injury. PLoS One 9:e103402. Heart
Skeletal muscle
Hunter 2014 PhD Thesis 2014 Hunter FW (2014) Old target; new paradigm: Determinants of sensitivity to hypoxia-directed anticancer prodrugs. PhD Thesis:1-353. CHO Other mammals
Pham 2014 Am J Physiol 2014 Pham T, Loiselle D, Power A, Hickey AJ (2014) Mitochondrial inefficiencies and anoxic ATP hydrolysis capacities in diabetic rat heart. Am J Physiol 307:C499–507. Heart Rat
Gnaiger 2014 MitoPathways 2014
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp.
Skeletal muscle
Fibroblast Human
Kiss 2014 FASEB J 2014 Kiss G, Konrad C, Pour-Ghaz I, Mansour JJ, Németh B, Starkov AA, Adam-Vizi V, Chinopoulos C (2014) Diaphorases provide NAD+ in anoxia. FASEB J 28:1682-97. Liver Mouse
Chinopoulos 2014 Methods Enzymol 2014 Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. HEK Human
Krumschnabel 2014 Methods Enzymol 2014
O2k-Protocols contents
Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.
Nervous system Mouse
Hara 2013 Eur J Anaesthesiol 2013 Hara N, Karlsson M, Sjövall F, Hansson Magnus J, Elmér E, Uchino H (2013) Early brain mitochondrial dysfunction in a mouse model of sepsis: 7AP4‐9. Eur J Anaesthesiol 30,112-112. Nervous system Mouse
Reilly 2013 J Exp Biol 2013 Reilly BD, Hickey AJ, Cramp RL, Franklin CE (2013) Decreased hydrogen peroxide production and mitochondrial respiration in skeletal muscle but not cardiac muscle of the green-striped burrowing frog, a natural model of muscle disuse. J Exp Biol 217:1087-93. Heart
Skeletal muscle
Magnesium Green 2013 Chinopoulos C (2013) Use of Magnesium Green 5N hexapotassium salt to measure ADP-ATP exchange rates. Mitochondr Physiol Network 2013-02-14.
Iftikar 2013 PLoS One 2013 Iftikar FI, Hickey AJ (2013) Do mitochondria limit hot fish hearts? Understanding the role of mitochondrial function with heat stress in Notolabrus celidotus. PLoS One 8:e64120. Heart Fishes
Jelenik 2013 Eur Heart J 2013 Jelenik T, Floegel U, Phielix E, Kaul K, Nowotny P, Partke HP, Schrader J, Roden M, Szendroedi S (2013) Non-alcoholic fatty liver disease and insulin resistance are associated with increased cardiac oxidative stress in mice. Eur Heart J 34:P5045. Heart Mouse
Hickey 2012 J Comp Physiol B 2012 Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182:91-100. Heart Fishes
Gnaiger 2012 Mitochondr Physiol Network Bioblast 2012 2012
Gnaiger E, Meissner B, Laner V, eds (2012) Bioblast 2012: Mitochondrial Competence. Mitochondr Physiol Network 17.12. OROBOROS MiPNet Publications, Innsbruck:96 pp. ISBN 978-3-9502399-5-9
Hunter 2012 Biochem Pharmacol 2012 Hunter FW, Wang J, Patel R, Hsu HL, Hickey AJ, Hay MP, Wilson WR (2012) Homologous recombination repair-dependent cytotoxicity of the benzotriazine di-N-oxide CEN-209: Comparison with other hypoxia-activated prodrugs. Biochem Pharmacol 83:574–85. CHO

O2k-Publications in the MiPMap

O2k-Publications: O2k-Fluorometry - Abstracts

Sort in ascending/descending order by a click on one of the small symbols in squares below.
Was submitted in year Has title Tissue and cell Cell line Mammal and model Nonmammal
Gnaiger 2016 Abstract SFRR Australasia 2016 2016 Facts and artefacts in measurements of H2O2 production under hypoxia. Saccharomyces cerevisiae
Chicco 2016a Abstract MitoFit Science Camp 2016 2016 Oxygen dependence of H2O2 release from skeletal muscle and cardiac mitochondria: influence of sample preparation. Heart
Skeletal muscle
Krumschnabel 2016b Abstract MitoFit Science Camp 2016 2016 O2k-MultiSensor: Mitochondrial respiration media for HRR and simultaneous O2k-Fluorometry. Kidney HEK Human
Ali 2015 Abstract MiP2015 2015 Mitochondrial function and ROS homeostasis in young mouse heart and brain: the sex factor! Heart
Nervous system
Doerrier 2015 Abstract MiPschool Greenville 2015 2015 "Succinate paradox" study by high resolution respirometry combined with fluorometry and spectrofluorometry: NextGen-O2k. Heart Mouse
Laner 2015 Abstract MiPschool Cape Town 2015 2015 The OROBOROS Oxygraph-2k for high-resolution respirometry and O2k-Fluorometry: a new quality in OXPHOS analysis. Heart Mouse
Saccharomyces cerevisiae
Rohlena 2015 Abstract MiP2015 2015 Ubiquinone-binding site mutagenesis reveals the role of mitochondrial complex II in cell death initiation. Other cell lines Human
Gnaiger 2015 Abstract MiP2015 2015 Mitochondrial function of cyropreserved HEK 293T cells: development of a reference sample for high-resolution respirometry. HEK Human
Sumbalova 2015 Abstract MiP2015 2015 High-resolution measurement of mitochondrial membrane potential and respiration – comparison of potentiometric and fluorometric methods. Nervous system Mouse
Doerrier 2015 Abstract MiP2015 2015 Simultaneous measurement of mitochondrial respiration, hydrogen peroxide production, and NADH autofluorescence to assess mitochondrial function. Heart Mouse
Abdel-Rahman 2015 Abstract MiPschool Cape Town 2015 2015 Assessment of NADPH-dependent oxygen consumption and hydrogen peroxide production by high-resolution respirometry and EPR spectroscopy. Nervous system Human
Smenes 2015 Abstract IOC100 2015 Smenes BT, Baekkerud FH, Hassel E, Wohlwend M, Slagsvold KH, Rognmo O, Wahba A (2015) Mitochondrial function in patients exercising prior to coronary surgery – A single blinded randomized controlled trial. Mitochondr Physiol Network 20.01. Skeletal muscle Human
Hoppel 2015 Abstract MiPschool London 2015 2015 Human blood cell types as study models of mitochondrial respiration and H2O2 production. Blood cells Human
Bezuidenhout 2015 Abstract MiPschool Cape Town 2015 2015 A study of the effects of fructose feeding & exercise on SIRT 3 regulation in skeletal muscle. Rat
Madlala 2015 Abstract MiPschool Cape Town 2015 2015 Fructose-induced ROS production: The role of uric acid and transforming growth factor (TGF)-β1. Liver Other cell lines Human
Meszaros 2014 Abstract MiP2014 2014 Methodological aspects of assessing mitochondrial function in small intestinal mucosal samples of rats and guinea pigs. Rat
Guinea pig
Perry 2014 Abstract MiP2014 2014 Evaluation of critical experimental parameters for assessing mitochondrial bioenergetics in permeabilized myofibres. Skeletal muscle Human
Power 2014 Abstract MiP2014 2014 The hot heart: cardiac mitochondrial energetics during hyperthermia. Heart Rat
Kluckova 2014 Abstract MiP2014 2014 Proximal ubiquinone binding site in mitochondrial respiratory Complex II is a target for vitamin E analog MitoVES, an experimental anti-cancer agent. Mitochondr Physiol Network 19.13. Other cell lines
Hickey 2014 Abstract MiP2014 2014 Mitochondrial inefficiencies and anoxic ATP hydrolysis capacities in diabetic rat heart. Heart Rat
Komlodi 2014 Abstract MiP2014 2014 Methylenblue, an enhancer of mitochondrial substrate-level phosphorylation. Nervous system Guinea pig
Krumschnabel 2014 Abstract MiP2014 2014 Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Nervous system Mouse
Laner 2013 ASMRM 2013 Laner V, Gradl P, Eigentler A, Fontana-Ayoub M, Fasching M, Gnaiger E (2013) The OROBOROS Oxygraph-2k for high-resolution respirometry and O2k-Fluorometry: a new quality in OXPHOS analysis. ASMRM2013. Heart Mouse
Jezek 2013 Abstract MiP2013 2013 Ježek P, Ježek J, Zelenka J, Dlasková A, Jabůrek M (2013) Antioxidant synergy of mitochondrial uncoupling protein UCP2 and phospholipase iPLA2γ. Mitochondr Physiol Network 18.08. Lung;gill
Islet cell;pancreas;thymus
Krumschnabel 2013 Abstract MiP2013 2013 Krumschnabel G, Eigentler A, Fontana-Ayoub M, Draxl A, Fasching M, Gnaiger E (2013) Tissue homogenates for respiratory OXPHOS analysis in comparative mitochondrial physiology: mouse and trout – heart and liver. Mitochondr Physiol Network 18.08. Heart
Nervous system
Mouse Fishes
Kressig 2013 Abstract IOC79 2013 Kressig F (2013) et al. Mechanism of increased pulmonary vascular remodeling in UCP2 k/o mice. Mitochondr Physiol Network 18.07 Lung;gill Other cell lines Mouse
Hickey 2013 Abstract MiP2013 2013 Hickey AJ, Iftikar FI, MacDonald J, Baker D (2013) Mitochondria in a changing climate? The role of mitochondrial in hyperthermic heart failure in different fish species. Mitochondr Physiol Network 18.08. Heart Other mammals Fishes
Cervinkova 2012 Abstract Bioblast 2012 Cervinkova Z, Stankova P, Cervinka M, Kucera O, Drahota Z (2012) Evaluation of mitochondrial functions – an important tool for experimental hepatology. Mitochondr Physiol Network 17.12. Liver Rat
Fasching 2012 Abstract Bioblast 2012 Fasching M, Sumbalova Z, Gnaiger E (2012) O2k-Fluorometry - a MitoCom project. Mitochondr Physiol Network 17.12. Nervous system Mouse
Kiss 2012 Abstract Bioblast 2012 Kiss G, Konrad C, Doczi J, Starkov AA, Kawamata H, Manfredi G, Zhang SF, Gibson GE, Beal MF, Adam-Vizi V, Chinopoulos C (2012) The negative impact of alpha-ketoglutarate dehydrogenase complex deficiency on matrix substrate-level phosphorylation. Mitochondr Physiol Network 17.12. Nervous system
Konrad 2012 Abstract Bioblast 2012 Konrad C, Kiss G, Torocsik B, Adam-Vizi V, Chinopoulos C (2012) Absence of Ca2+-induced mitochondrial permeability transition but presence of bongkrekate-sensitive nucleotide exchange in C. crangon and P. serratus. Mitochondr Physiol Network 17.12. Artemia Crustaceans
Horvath 2012 Abstract Bioblast 2012 Horvath G, Eigentler A, Gnaiger E, Tretter L (2012) Respiratory substrate effects on mitochondrial membrane potential - experiments with mouse cardiac muscle homogenate. Mitochondr Physiol Network 17.12. Heart Mouse
Stoeger 2012 Abstract Bioblast 2012 Stoeger H, Fontana-Ayoub M, Eigentler A, Gnaiger E (2012) Cell respiration with yeast as a model. Mitochondr Physiol Network 17.12. Saccharomyces cerevisiae Fungi
Fasching 2011 Abstract Berlin 2011 Fasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract Berlin. Nervous system Mouse
Sumbalova 2011 Abstract Kagoshima 2011 Sumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H2O2 production, and the upper limit of mitochondrial performance. Abstract Kagoshima. Nervous system Mouse
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