O2k-Publications: Instruments;methods

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O2k-Publications: Instruments;methods

O2k-Publications in the MiPMap

»O2k-Publications: Topics

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 YearReference
MiPNet12.23 FibreRespiration2019-01-10
O2k-Protocols contents
Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle.
MiPNet22.16 DatLab 7 Innovations: DL-Protocols2018-11-26
O2k-Protocols
DatLab 7.3 Innovations: DL-Protocols
MiPNet23.12 DL-Protocols help2018-11-26
O2k-Protocols
DatLab Protocols: Help DatLab 7.
MiPNet20.14 AmplexRed H2O2-production2018-11-26
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed.
MiPNet20.13 Safranin mt-membranepotential2018-09-25
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.
MiPNet23.15 O2k-pH ISE-Module2018-09-08
O2k-Manual
Measurement of proton flux with the O2k-pH ISE-Module.
MiPNet14.06 Instrumental O2 background2018-08-07
O2k-Protocols
O2k Quality Control 2: Instrumental oxygen background correction and accuracy of oxygen flux.
MiPNet21.16 DatLab 7 Innovations2018-07-12
About Oroboros
DatLab 7: innovations.
MiPNet21.06 SUIT RP2018-06-25
O2k-Protocols
SUIT reference protocol for OXPHOS analysis by high-resolution respirometry.
MiPNet18.10 O2k specifications2018-05-04
Oroboros
O2k-FluoRespirometer: specifications for respirometry and comprehensive OXPHOS analysis.
MiPNet21.17 BloodCellsIsolation2018-04-09
O2k-Protocols
O2k-Protocols: Isolation of blood cells for HRR.
MiPNet06.03 POS-calibration-SOP2018-01-07
O2k-Protocols
O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.
Fisher-Wellman 2018 Cell Rep2018Fisher-Wellman KH, Davidson MT, Narowski TM, Lin CT, Koves TR, Muoio DM (2018) Mitochondrial diagnostics: A multiplexed assay platform for comprehensive assessment of mitochondrial energy fluxes. Cell Rep 24:3593-606.
Ramil 2018 Thesis2018Ramil LR, Gulbinas A, Cesna V (2018) “HIPEC” hyperthermic intraperitoneal chemotherapy. Evaluation of intraperitoneal free cancer cells. Master's Thesis.
Doerrier 2018 Methods Mol Biol2018Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70.
Van Daele 2018 Medicine (Baltimore)2018Van Daele E, Van Nieuwenhove Y, Ceelen W, Vanhove C, Braeckman BP, Hoorens A, Van Limmen J, Varin O, Van de Putte D, Willaert W, Pattyn P (2018) Assessment of graft perfusion and oxygenation for improved outcome in esophageal cancer surgery: Protocol for a single-center prospective observational study. Medicine (Baltimore) 97:e12073.
Deschaseaux 2018 US Patent2018Deschaseaux F, Guilloton F, Muller S, Sensebe L, Casteilla L, Carriere-Pazat A (2018) Method for obtaining human brown/beige adipocytes . US Patent US20180216070A1.
Havird 2018 bioRxiv2018Havird JC, Noe GR, Link L, Torres A, Logan DC, Sloan DB, Chicco AJ (2018) Assessing mitochondrial function in angiosperms with highly divergent mitochondrial genomes. bioRxiv doi: https://doi.org/10.1101/448902.
Puurand 2018 Int J Mol Sci2018Puurand M, Tepp K, Klepinin A, Klepinina L, Shevchuk I, Kaambre T (2018) Intracellular energy-transfer networks and high-resolution respirometry: a convenient approach for studying their function. Int J Mol Sci 19:e2933.
Smirnova 2018 Sci Rep2018Smirnova IA, Ädelroth P, Brzezinski P (2018) Extraction and liposome reconstitution of membrane proteins with their native lipids without the use of detergents. Sci Rep 8:14950.
Dias 2018 Anal Biochem2018Dias C, Lourenco CF, Barbosa RM, Laranjinha J, Ledo A (2018) Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices. Anal Biochem 551:43-50.
Komlodi 2018 Methods Mol Biol2018Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55.
Fischer 2018 BMC Musculoskelet Disord2018Fischer MJ, Horvath G, Krismer M, Gnaiger E, Goebel G, Pesta DH (2018) Evaluation of mitochondrial function in chronic myofascial trigger points - a prospective cohort pilot study using high-resolution respirometry. BMC Musculoskelet Disord 19:388.
MiPNet19.03 O2k-cleaning and ISS2017-09-13
O2k-Protocols
O2k-Chamber cleaning SOP and Integrated Suction System (ISS).
MiPNet19.14 SOP Hamilton microsyringes2017-08-03
O2k-Protocols
SOP for manual O2k-titrations with Hamilton microsyringes.
MiPNet15.09. Yeast: HRR Reference Assay2017-02-05
O2k-Protocols
A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast.
Djafarzadeh 2017 J Vis Exp2017Djafarzadeh S, Jakob SM (2017) High-resolution respirometry to assess mitochondrial function in permeabilized and intact cells. J Vis Exp 8.doi: 10.3791/54985..
Westerlund 2017 Pediatr Res2017Westerlund E, Marelsson SE, Ehinger JK, Sjövall F, Morota S, Åsander Frostner E, Oldfors A, Darin N, Lundgren J, Hansson MJ, Fellman V, Elmér E (2017) Oxygen consumption in platelets as an adjunct diagnostic method for pediatric mitochondrial disease. Pediatr Res 83:455-65.
Djafarzadeh 2017b J Vis Exp2017Djafarzadeh S, Jakob SM (2017) Isolation of intact mitochondria from skeletal muscle by differential centrifugation for High-Resolution Respirometry measurements. J Vis Exp doi: 10.3791/55251.
Vienne 2017 Anal Biochem2017Vienne JC, Cimetta C, Dubois M, Duburcq T, Favory R, Dessein AF, Fontaine M, Joncquel-Chevalier Curt M, Cuisset JM, Douillard C, Mention-Mulliez K, Dobbelaere D, Vamecq J (2017) A fast method for high resolution oxymetry study of skeletal muscle mitochondrial respiratory chain complexes. Anal Biochem 528:57-62.
MiPNet08.09 CellRespiration2016-08-11
O2k-Procedures contents
High-resolution respirometry and coupling control protocol with intact cells: ROUTINE, LEAK, ET-pathway, ROX.
MiPNet17.05 O2k-Fluo LED2-Module2016-08-09
O2k-Manual
O2k-Fluo LED2-Module.
MiPNet19.18A O2k-Series G: Start2016-08-08
O2k-Manual
Oxygraph-2k: start high-resolution respirometry.
MiPNet19.18C DatLab 6: Guide2016-08-08
O2k-Manual
DatLab-guide through the menus.
MiPNet19.18 O2k-Series G Core manual2016-08-08
O2k-Manual
O2k-Core manual contents.
MiPNet19.18B POS-service2016-08-08
O2k-Manual
Service of the polarographic oxygen sensor OroboPOS.
MiPNet19.18E O2 flux analysis - DatLab 62016-08-08
O2k-Manual
Oxygen flux analysis: DatLab real-time.
MiPNet10.04 CellRespiration2016-08-08
O2k-Protocols
An experiment with high-resolution respirometry: coupling control in cell respiration.
MiPNet20.04 O2k-checklist2016-08-05
O2k-Protocols
O2k-checklist: get started with an O2k-experiment.
MiPNet21.07 MitoFit DatLab PT2016-07-20
O2k-Protocols
MitoFit DatLab proficiency test.
Osiki 2016 FASEB J2016Osiki PO, Ojuka E, Marmaan G (2016) An investigation of the suitability of using octanoylcarnitine together with malate as a substrate combination for assessing Beta-oxidation using High Resolution Respirometry. FASEB J 30: 1015.12 .
Jang 2016 J Intensive Care Med2016Jang DH, Greenwood JC, Spyres MB, Eckmann D (2016) Measurement of mitochondrial respiration and motility in acute care: sepsis, trauma, and poisoning. J Intensive Care Med 32:86-94.
MiPNet21.12 Comparison mt respiration media2016
O2k-Protocols
O2k-MultiSensor: Mitochondrial respiration media for HRR and simultaneous O2k-Fluorometry.
Ojuka 2016 Am J Physiol Endocrinol Metab2016Ojuka E, Andrew B, Bezuidenhout N, George S, Maarman G, Madlala HP, Mendham A, Osiki PO (2016) Measurement of β-oxidation capacity of biological samples by respirometry: a review of principles and substrates. Am J Physiol Endocrinol Metab 310:E715-23.
MiPNet21.13 Comparison mt-preparations2016
O2k-Protocols
O2k-Protocols: Mitochondrial preparations for HRR.
Salin 2016 Physiol Rep2016Salin K, Villasevil EM, Auer SK, Anderson GJ, Selman C, Metcalfe NB, Chinopoulos C (2016) Simultaneous measurement of mitochondrial respiration and ATP production in tissue homogenates and calculation of effective P/O ratios. Physiol Rep 10.14814/phy2.13007.
MiPNet21.14 Reference sample HRR2016
O2k-Protocols
Development of a reference sample for HRR.
Lark 2016 Am J Physiol Cell Physiol2016Lark DS, Torres MJ, Lin CT, Ryan TE, Anderson EJ, Neufer PD (2016) Direct real-time quantification of mitochondrial oxidative phosphorylation efficiency in permeabilized skeletal muscle myofibers. Am J Physiol Cell Physiol 311:C239-45.
Fisar 2016a Folia Biol (Praha)2016Fisar Z, Hroudova J (2016) Pig brain mitochondria as a biological model for study of mitochondrial respiration. Folia Biol (Praha) 62:15-25.
Stier 2016 Methods Ecol Evol2016Stier A, Romestaing C, Schull Q, Lefol E, Robin JP, Roussel D, Bize P (2016) How to measure mitochondrial function in birds using red blood cells: a case study in the king penguin and perspectives in ecology and evolution. Methods Ecol Evol doi:10.1111/2041-210X.12724.
Khattar 2016 J Neurosci Methods2016Khattar NK, Yablonska S, Baranov SV, Baranova OV, Kretz ES, Larkin TM, Carlisle DL, Richardson RM, Friedlander RM (2016) Isolation of functionally active and highly purified neuronal mitochondria from human cortex. J Neurosci Methods 263:1-6.
Dechandt 2016 Anal Biochem2016Dechandt CRP, Couto-Lima CA, Alberici LC (2016) Triglyceride depletion of brown adipose tissue enables analysis of mitochondrial respiratory function in permeabilized biopsies. Anal Biochem 515:55-60.
Bai 2016 PLOS ONE2016Bai F, Fink BD, Yu L, Sivitz WI (2016) Voltage-dependent regulation of Complex II energized mitochondrial oxygen flux. PLOS ONE 11:e0154982.
Ruas 2016 PLOS ONE2016Ruas JS, Siqueira-Santos ES, Amigo I, Rodrigues-Silva E, Kowaltowski AJ, Castilho RF (2016) Underestimation of the maximal capacity of the mitochondrial electron transport system in oligomycin-treated cells. PLOS ONE 11:e0150967.
Reich 2016 Thesis2016Reich V (2016) Pathophysiologie und Verfahrensentwicklung: Mechanismen der Metabolisierung von Schwefelwasserstoff im mitochondrialen Atmungskreislauf von Lungenalveolarmakrophagen. Dissertation p91.
MiPNet19.18D O2k-Series G and DatLab 6: Calibration2015-08-08
O2k-Protocols
O2k-calibration by DatLab 6.
MiPNet17.03 Shredder vs Fibres2015-06-18
O2k-Protocols
Mitochondrial respiration in permeabilized fibres versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver2015-06-16
O2k-Protocols
Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver.
MiPNet17.16 O2k-Spectrophotometry2015-06-16
O2k-Protocols
O2k-Spectrophotometry - A MitoCom Project.
MiPNet06.06 Chemical O2 background2015-04-27
O2k-Protocols
Oxygraph assay of cytochrome c oxidase activity: chemical background correction.
Chowdhury 2015 Biosci Rep2015Chowdhury SK, Djordjevic J, Albensi B, Fernyhough P (2015) Simultaneous evaluation of substrate-dependent oxygen consumption rates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep 36:e00286.
Cagnone 2015 Methods Mol Biol2015Cagnone G, Vaghjiani V, Lee W, Sun C, Johnson J, Yeung KY, St John JC (2015) Analysis of the mitochondrial DNA and its replicative capacity in induced pluripotent stem cells. Methods Mol Biol 1357:231-67.
Kluckova 2015 Methods Mol Biol2015Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J (2015) Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents. Methods Mol Biol 1265:181-94.
Harrison 2015 J Appl Physiol2015Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol 119:1210-8.
Abou-Hamdan 2015 Methods Enzymol2015Abou-Hamdan A, Guedouari-Bounihi H, Lenoir V, Andriamihaja M, Blachier F, Bouillaud F (2015) Oxidation of H2S in mammalian cells and mitochondria. Methods Enzymol 554:201-28.
MiPNet20.17 MiP2015 Lucni Bouda2015
MiPsociety
Pecina P et al, eds (2015) Mitochondrial physiology conference. MiP2015. Mitochondr Physiol Network 20.17:87 pp.
Wuest 2015 J Physiol2015Wüst RC, Helmes M, Stienen GJ (2015) Rapid changes in NADH and flavin autofluorescence in rat cardiac trabeculae reveal large mitochondrial complex II reserve capacity. J Physiol 593:1829-40.
Krumschnabel 2015 Methods Mol Biol2015
O2k-Protocols contents
Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61.
Canto 2015 Curr Protoc Mouse Biol2015Cantó C, Garcia-Roves PM (2015) High-resolution respirometry for mitochondrial characterization of ex vivo mouse tissues. Curr Protoc Mouse Biol 5:135-53.
Makrecka-Kuka 2015 Biomolecules2015
O2k-Protocols contents
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38.
Weissig 2015 Methods Mol Biol II2015Weissig V, Edeas M, eds (2015) Mitochondrial medicine: Volume II, Manipulating mitochondrial function. Methods Mol Biol 1265:1-447.
Angiulli 2015 Biochim Biophys Acta2015Angiulli G, Lantella A, Forte E, Angelucci F, Colotti G, Ilari A, Malatesta F (2015) Leishmania infantum trypanothione reductase is a promiscuous enzyme carrying an NADPH:O2 oxidoreductase activity shared by glutathione reductase. Biochim Biophys Acta 1850:1891-7.
Kake-Guena 2015 J Exp Zool A Ecol Genet Physiol2015Kake-Guena SA, Touisse K, Vergilino R, Dufresne F, Blier PU, Lemieux H1 (2015) Assessment of mitochondrial functions in Daphnia pulex clones using high-resolution respirometry. J Exp Zool A Ecol Genet Physiol 323:292-300.
MiPNet12.10 TIP2k-manual2014-03-06
O2k-Manual
Titration-Injection microPump. TIP2k user manual.
MiPNet15.03 O2k-MultiSensor-ISE2014-03-06
O2k-Manual
O2k-MultiSensor system with ion selective electrodes (ISE).
MiPNet15.05 NO-manual2014-03-06
O2k-Manual
O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2.
MiPNet19.01 O2k-Core Manual Series F DatLab 52014-03
O2k-Manual
O2k-Core Manual - up to O2k-Series F and DatLab 5.
MiPNet12.11 MitoRespiration2014-02-20
O2k-Protocols
Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.
Schiffer 2014 FASEB J2014Schiffer TA, Ekblom B, Lundberg JO, Weitzberg E, Larsen FJ (2014) Dynamic regulation of metabolic efficiency explains tolerance to acute hypoxia in humans. FASEB J 28:4303-11.
Ryan 2014 J Physiol2014Ryan TE, Brophy P, Lin CT, Hickner RC, Neufer PD (2014) Assessment of in vivo skeletal muscle mitochondrial respiratory capacity in humans by near-infrared spectroscopy: a comparison with in situ measurements. J Physiol 592:3231-41.
Krumschnabel 2014 Methods Enzymol2014
O2k-Protocols contents
Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.
Larsen 2014 Anal Biochem2014Larsen S, Kraunsøe R, Gram M, Gnaiger E, Helge JW, Dela F (2014) The best approach: homogenization or manual permeabilization of human skeletal muscle fibers for respirometry? Anal Biochem 446:64-8.
Herbst 2014 J Physiol2014Herbst EA, Paglialunga S, Gerling C, Whitfield J, Mukai K, Chabowski A, Heigenhauser GJ, Spriet LL, Holloway GP (2014) Omega-3 supplementation alters mitochondrial membrane composition and respiration kinetics in human skeletal muscle. J Physiol 592:1341-52.
Van Bergen 2014 Mitochondrion2014Van Bergen NJ, Blake RE, Crowston JG, Trounce IA (2014) Oxidative phosphorylation measurement in cell lines and tissues. Mitochondrion 15:24-33.
Pecina 2014 Biochim Biophys Acta Clinical2014Pecina P, Houstkova H, Mracek T, Pecinova A, Nuskova H.Tesarova M, Hansikova H, Janota J, Zemanc J, Houstek J (2014) Noninvasive diagnostics of mitochondrial disorders in isolated lymphocytes with high resolution respirometry. Biochim Biophys Acta Clinical 2:62–71.
Gnaiger 2014 MitoPathways2014
O2k-Protocols
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp.
Chinopoulos 2014 Methods Enzymol2014Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48.
Chakraborty 2014 J Cardiovasc Dis2014Chakraborty M, Phillips ARJ, Macdonald J, Windsor JA, Hickey AJR (2014) Mitochondrial respiration in mononuclear cells and heart fibers in spontaneously hypertensive rats. J Cardiovasc Dis ISSN:2330-459.
Karabatsiakis 2014 Transl Psychiatry2014Karabatsiakis A, Böck C, Salinas-Manrique J, Kolassa S, Calzia E, Dietrich DE, Kolassa IT (2014) Mitochondrial respiration in peripheral blood mononuclear cells correlates with depressive subsymptoms and severity of major depression. Transl Psychiatry 4:e397.
Laner 2014 Mitochondr Physiol Network MiP20142014
MiPsociety
Laner V, Gnaiger E, eds (2014) Mitochondrial physiology – methods, concepts and biomedical perspectives. MiP2014. Mitochondr Physiol Network 19.13:88 pp. ISBN 978-3-9502399-9-7
Sjoevall 2013 Mitochondrion2013Sjövall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, Arnbjörnsson E, Hansson Magnus J, Fellman V, Elmér E (2013) Mitochondrial respiration in human viable platelets - methodology and influence of gender, age and storage. Mitochondrion 13:7-14.
Chu 2013 PLoS One2013Chu MJ, Phillips AR, Hosking AW, Macdonald JR, Bartlett AS, Hickey AJ (2013) Hepatic mitochondrial function analysis using needle liver biopsy samples. PLoS One 8:e79097.
Magnesium Green2013Chinopoulos C (2013) Use of Magnesium Green 5N hexapotassium salt to measure ADP-ATP exchange rates. Mitochondr Physiol Network 2013-02-14.
Kettunen 2013 Thesis University of Applied Sciences - Turku2013Kettunen J (2013) Ruskean rasvan hapenkulutuksen mittaaminen. Thesis University of Applied Sciences - Turku pp106.
Perry 2013 Diabetes2013Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62:1041-53.
Pesta 2012 Methods Mol Biol2012
O2k-Protocols
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58.
Larsen 2012 CryoLetters2012Larsen S, Wright-Paradis C, Gnaiger E, Helge JW, Boushel RC (2012) Cryopreservation of human skeletal muscle impairs mitochondrial function. Cryo Letters 33:170-6.
Gnaiger 2012 Mitochondr Physiol Network Bioblast 20122012
Oroboros
Gnaiger E, Meissner B, Laner V, eds (2012) Bioblast 2012: Mitochondrial Competence. Mitochondr Physiol Network 17.12. Oroboros MiPNet Publications, Innsbruck:96 pp. ISBN 978-3-9502399-5-9
Horan 2012 J Gerontol A Biol Sci Med Sci2012Horan MP, Pichaud N, Ballard JWO (2012) Review: Quantifying mitochondrial dysfunction in complex diseases of aging. J Gerontol A Biol Sci Med Sci 67:1022-35.
MiPNet08.12 IOC222011-12-11
O2k-Protocols
NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.
MiPNet12.17 BovineHeartMito2011-12-11
O2k-Protocols contents
Mitochondrial respiration with frozen bovine heart mitochondria: diagnostic tests.
MiPNet15.08 TPP electrode2011-12-11
O2k-Protocols
Ion selective electrode for TPP+ and high-resolution respirometry.
Hench 2011 PloS One2011Hench J, Bratic Hench I, Pujol C, Ipsen S, Brodesser S, Mourier A, Tolnay M, Frank S, Trifunovic A (2011) A tissue-specific approach to the analysis of metabolic changes in Caenorhabditis elegans. PloS One 6:e28417.
MiPNet12.20 O2k-calibration tutorial2010-10-19
O2k-Protocols
O2k: Oxygen calibration and O2k-background tutorial.
MiPNet06.05 Specifications2010
Logo OROBOROS INSTRUMENTS.jpg
Gnaiger E (2010) Test experiments on specifications of the Oroboros Oxygraph. Mitochondr Physiol Network 6.5.
Scandurra 2010 Adv Exp Med Biol2010Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25.
Sommer 2010 Eur Respir J2010Sommer N, Pak O, Schörner S, Derfuss T, Krug A, Gnaiger E, Ghofrani HA, Schermuly RT, Huckstorf C, Seeger W, Grimminger F, Weissmann N (2010) Mitochondrial cytochrome redox states and respiration in acute pulmonary oxygen sensing. Eur Respir J 36:1056-66.
Komary 2010 Biochim Biophys Acta2010Komary Z, Tretter L, Adam-Vizi V (2010) Membrane potential-related effect of calcium on reactive oxygen species generation in isolated brain mitochondria. Biochim Biophys Acta 1797:922-8.
Benani 2009 J Neurosci Methods2009Benani A, Barquissau V, Carneiro L, Salin B, Colombani A-L, Leloup C, Casteilla L, Rigoulet M, Pénicaud L (2009) Method for functional study of mitochondria in rat hypothalamus. J Neurosci Methods 178:301-7.
Lanza 2009 Methods Enzymol2009Lanza IR, Nair KS (2009) Functional assessment of isolated mitochondria in vitro. Methods Enzymol 457:349-72.
Rostrup M 2008 Amino Acids2008Rostrup M, Fossbakk A, Hauge A, Kleppe R, Gnaiger E, Haavik J (2008) Oxygen dependence of tyrosine hydroxylase. Amino Acids 34:455-64.
Gnaiger 2008 POS2008
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.
Park 2007 Sensors IEEE2007Park J, Chang J, Choi M, Pak JJ, Lee DY, Pak YK (2007) Microfabricated Clark-type sensor for measuring dissolved oxygen. Sensors IEEE: 1412-1415.
Labajova 2006 Anal Biochem2006Labajova A, Vojtiskova A, Krivakova P, Kofranek J, Drahota Z, Houstek J (2006) Evaluation of mitochondrial membrane potential using a computerized device with a tetraphenylphosphonium-selective electrode. Anal Biochem 353:37-42.
Huetter 2006 Exp Gerontol2006Hütter E, Unterluggauer H, Garedew A, Jansen-Dürr P, Gnaiger E (2006) High-resolution respirometry - a modern tool in aging research. Exp Gerontol 41:103-9.
Mukhanov 2006 Thermoc Acta2006Mukhanov VS, Kemp RB (2006) Simultaneous photocalorimetric and oxygen polarographic measurements on Dunaliella maritima cells reveal a thermal discrepancy that could be due to nonphotochemical quenching. Thermochimica Acta 446:11-19.
Garedew 2005 Eur Shock Soc2005Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The Oroboros O2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna:107-11.
Doeller 2005 Anal Biochem2005Doeller JE, Isbell TS, Benavides G, Koenitzer J, Patel H, Patel RP, Lancaster JR Jr, Darley-Usmar VM, Kraus DW (2005) Polarographic measurement of hydrogen sulfide production and consumption by mammalian tissues. Anal Biochem 341:40-51.
Kraft 2004 Thesis Universität Halle-Wittenberg - Germany2004Kraft A (2004) Langzeitstabilität der Mitochondrienfunktion permeabilisierter Muskelfasern in der Diagnostik mitochondrialer Enzephalomyopathien. Thesis Universität Halle-Wittenberg - Germany 74pp.
Kalbacova 2003 Cytometry2003Kalbácová M, Vrbacký M, Drahota Z, Melková Z (2003) Comparison of the effect of mitochondrial inhibitors on mitochondrial membrane potential in two different cell lines using flow cytometry and spectrofluorometry. Cytometry 52A:110-6.
Gregori 20032002Gregori G, Denis M, Lefevre D, Beker B (2002) A flow cytometric approach to assess phytoplankton respiration. Methods Cell Sci 24 (1-3):99-106
Kuznetsov 2002 Anal Biochem2002Kuznetsov AV, Strobl D, Ruttmann E, Königsrainer A, Margreiter R, Gnaiger E (2002) Evaluation of mitochondrial respiratory function in small biopsies of liver. Anal Biochem 305:186-94.
Gnaiger 2001 Respir Physiol2001Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97.
MiPNet06.01 O2k-Overview2001
Oroboros
Gnaiger E (2001) The Oxygraph for high-resolution respirometry. Mitochondr Physiol Network 06.01.
Gnaiger 2000 Life in the Cold2000
O2k-Protocols contents
Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42.
MiPNet04.05 Titration-Injection1999-01
O2k-Protocols contents
From step titration to ramp injection: Uncoupling by FCCP with TIP.
Boveris 1999 Methods Enzymol1999Boveris A, Costa LE, Cadenas E, Poderoso JJ (1999) Regulation of mitochondrial respiration by ADP, O2 and NO. Methods Enzymol 301:188-98.
Gnaiger 1998 BTK-HRR1998Gnaiger E, Kuznetsov AV, Lassnig B, Fuchs A, Reck M, Renner K, Stadlmann S, Rieger G, Margreiter R (1998) High-resolution respirometry. Optimum permeabilization of the cell membrane by digitonin. In BioThermoKinetics in the Post Genomic Era (Larsson C, Pahlman I-L, Gustafsson L, eds) Chalmers Reproservice, Göteborg:89-95.
Gnaiger 1998 Biochim Biophys Acta1998Gnaiger E, Lassnig B, Kuznetsov AV, Margreiter R (1998) Mitochondrial respiration in the low oxygen environment of the cell: Effect of ADP on oxygen kinetics. Biochim Biophys Acta 1365:249-54.
Kuznetsov 1998 BTK1998Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Diffusion limitation of oxygen versus ADP in permeabilized muscle fibers. In BioThermoKinetics in the Post Genomic Era (Larsson C, Påhlman I-L, Gustafsson L, eds) Chalmers Reproservice, Göteborg:273-6.
MiPNet02.05 DatLab2 O2Kinetics1997-01
O2k-Manual
DatLab 2. Analysis of oxygen kinetics.
Gnaiger 1995 J Bioenerg Biomembr1995Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96.
Haller 1994 Anal Biochem1994Haller T, Ortner M, Gnaiger E (1994) A respirometer for investigating oxidative cell metabolism. Toward optimization of respiratory studies. Anal Biochem 218:338-42.
Mendez 1994 BTK-1911994
BTK1994
Méndez G, Gnaiger E (1994) How does oxygen pressure control oxygen flux in isolated mitochondria? A methodological approach by high-resolution respirometry and digital data analysis. In: What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermo-Kinetics 3. Innsbruck Univ Press:191-4.
MiPNet03.03 Internat-Oxygraph-Workshops1991-
O2k-Workshops.png
Innsbruck AT, since 1991. International oxygraph workshops on high-resolution respirometry, IOC.
  • Gnaiger E, Forstner H, eds (1983) Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Springer, Berlin, Heidelberg, New York: 370 pp. »Contents


O2k-Publications in the MiPMap

O2k-Publications: Instruments; methods - Abstracts

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 YearReference
Chang 2018 Life Sciences Meeting 2018 Innsbruck AT2018pH dependence of mitochondrial respiration and H2O2 production in oral cancer cells – a pilot study.
Garcia-Roves 2018 MiP2018b2018
Pablo Garcia-Roves
Generating mitochondrial respirometry reference values from permeabilized mouse soleus muscle fibers. Working Group 2 report. Garcia-Roves_Presentation
Pranger 2018 Emerging Concepts in Mitochondrial Biology2018
PrangerF.JPG
High-resolution respirometry with a reduced chamber volume – which are the compromises in OXPHOS analysis?
Sobotka 2018 MiP20182018
Ondrej Sobotka
Measurement of ROS production under hypoxia and unexpected methodological pitfalls of Amplex UltraRed assay.
Krajcova 2018 MiPschool Tromso E22018
Adela Krajcova
High-resolution respirometry to assess function of mitochondria in native homogenates of human heart muscle.
Garcia-Souza 2018 Life Sciences Meeting 2018 Innsbruck AT2018A respirometric cell viability test for peripheral-blood mononuclear cells and platelets.
Huete-Ortega 2018 MiPschool Tromso E22018
Maria Huete-Ortega
High-resolution respirometry: new perspectives for the study of bioenergetics in algae and plants.
Keller 2018 MiP20182018
Markus A Keller
The structural molecular diversity of mitochondrial cardiolipins.
Ganetzky 2018 IOC1342018SUIT protocol development for zebrafish embryos.
Keppner 2018 MiP20182018
Gloria Keppner
Effects of cryopreservation of peripheral blood mononuclear cells.
Doerrier 2018 MiP2018b2018
Carolina Doerrier
Evaluation of anaplerotic pathways to avoid artefacts in respirometric measurement of fatty acid oxidation.
Doerrier 2018 MiP2018c2018
Carolina Doerrier
Inter-laboratory study on human permeabilized myofibres: effects of fibre preparation, respiration buffer, and blebbistatin on mitochondrial respiratory capacity and oxygen limitation.
Meszaros 2017 MiPschool Obergurgl2017
Andras Meszaros
(Very) High-resolution respirometry – a deep dive into oxygen dependence of mitochondrial respiration.
Laner 2016 Abstract MitoFit Science Camp 20162016OXPHOS and ET-capacity in permeabilized fibres of canine superathletes. Analysis of HRR data presenting unexpected challenges.
Sparagna 2016 Abstract IOC1092016Sparagna GC (2016) Measuring cardiac mitochondrial function in pediatric patients before and after implantation of a left ventricular assist device. Mitochondr Physiol Network 21.01.
Sumbalova 2016b Abstract MitoFit Science Camp 20162016Human blood cells: isolation and HRR.
Markova 2016 Abstract MitoFit Science Camp 20162016Porcine hearts samples preparation: PBI-Shredder or saponin?
Gnaiger 2016 Abstract SFRR Australasia 20162016Facts and artefacts in measurements of H2O2 production under hypoxia.
Krumschnabel 2016b Abstract MitoFit Science Camp 20162016O2k-MultiSensor: Mitochondrial respiration media for HRR and simultaneous O2k-Fluorometry.
Sumbalova 2016 Abstract Mito Xmas Meeting Innsbruck2016Methods for evaluation of mitochondrial membrane potential with multi-sensor high-resolution respirometry: potentiometry vs fluorometry.
Bezuidenhout 2016b Abstract MitoFit Science Camp 20162016Analysis and comparison of mitochondrial function using cooling plate or ice during mechanical separation of mouse skeletal muscle fibers.
Krumschnabel 2016 Abstract Mito Xmas Meeting Innsbruck2016Establishment of a reference sample for high-resolution respirometry.
Krumschnabel 2016a Abstract MitoFit Science Camp 20162016O2k-Protocols: Mitochondrial preparations for HRR.
Ehinger 2015 Abstract MiPschool London 20152015Cell-permeable mitochondrial complex II substrates – a new compound class for treatment of complex I-linked mitochondrial disease.
Abdel-Rahman 2015 Abstract MiPschool Cape Town 20152015Assessment of NADPH-dependent oxygen consumption and hydrogen peroxide production by high-resolution respirometry and EPR spectroscopy.
Shevchuk 2015 Abstract MiP20152015Analysis of energy fluxes in colorectal cancer saponin skinned tissues and Caco-2 cells.
Hoppel 2015 Abstract MiPschool London 20152015Human blood cell types as study models of mitochondrial respiration and H2O2 production.
Laner 2015 Abstract MiPschool Cape Town 20152015The Oroboros Oxygraph-2k for high-resolution respirometry and O2k-Fluorometry: a new quality in OXPHOS analysis.
Doerrier 2015 Abstract MiP20152015Simultaneous measurement of mitochondrial respiration, hydrogen peroxide production, and NADH autofluorescence to assess mitochondrial function.
Laner 2015 Abstract MiPschool Greenville 20152015Comparative mitochondrial physiology: OXPHOS and ET capacity in permeabilized fibres of canine superathletes.
Maarman 2015 Abstract MiPschool Cape Town 20152015Characterisation of mitochondrial function in a model of pulmonary hypertension-induced right ventricular failure.
Sumbalova 2015 Abstract MiP20152015High-resolution measurement of mitochondrial membrane potential and respiration – comparison of potentiometric and fluorometric methods.
Doerrier 2015 Abstract MiPschool Greenville 20152015"Succinate paradox" study by high resolution respirometry combined with fluorometry and spectrofluorometry: NextGen-O2k.
Mussulini 2015 Abstract MiPschool London2015Changes of brain substrates preference and metabolism during seizure induced by pentylenetetrazole in adult zebrafish.
Laner 2015 Abstract MiP20152015Comparative mitochondrial physiology: OXPHOS and ET capacity in permeabilized fibres of canine superathletes.
Gnaiger 2015 Abstract MiP20152015Mitochondrial function of cyropreserved HEK 293T cells: development of a reference sample for high-resolution respirometry.
Pettersen 2015 Abstract MiPschool London 20152015Metabolic adjustments after reversible induction of mitochondrial biogenesis via AMPK/NRF1 in HeLa cells.
Volani 2015 Abstract IOC1062015Volani C, Haschka D, Demetz E, Doerrier C, Gnaiger E, Weiss G (2015) Determination of mitochondrial respiration in peripheral blood mononuclear cells. Mitochondr Physiol Network 20.10.
Kaambre 2015 Abstract MiP20152015Quantitative characterization of respiratory parameters of human colorectal and breast cancer clinical material.
Ali 2014 Abstract MiP20142014Oxygen-consuming and ROS-producing activities in synaptosomes.
Meszaros 2014 Abstract MiP20142014Methodological aspects of assessing mitochondrial function in small intestinal mucosal samples of rats and guinea pigs.
Perry 2014 Abstract MiP20142014Evaluation of critical experimental parameters for assessing mitochondrial bioenergetics in permeabilized myofibres.
Sumbalova 2014 Abstract MiP20142014Optimization of malate concentration for high-resolution respirometry: mitochondria from rat liver and brain.
Laner 2013 ASMRM2013Laner V, Gradl P, Eigentler A, Fontana-Ayoub M, Fasching M, Gnaiger E (2013) The Oroboros O2k for high-resolution respirometry and O2k-Fluorometry: a new quality in OXPHOS analysis. ASMRM2013.
Krumschnabel 2013 Abstract MiP20132013Krumschnabel G, Eigentler A, Fontana-Ayoub M, Draxl A, Fasching M, Gnaiger E (2013) Tissue homogenates for respiratory OXPHOS analysis in comparative mitochondrial physiology: mouse and trout – heart and liver. Mitochondr Physiol Network 18.08.
Cervinkova 2012 Abstract Bioblast2012Cervinkova Z, Stankova P, Cervinka M, Kucera O, Drahota Z (2012) Evaluation of mitochondrial functions – an important tool for experimental hepatology. Mitochondr Physiol Network 17.12.
Fasching 2012 Abstract Bioblast2012Fasching M, Sumbalova Z, Gnaiger E (2012) O2k-Fluorometry - a MitoCom project. Mitochondr Physiol Network 17.12.
Sumbalova 2011 Abstract Kagoshima2011Sumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H2O2 production, and the upper limit of mitochondrial performance. Abstract Kagoshima.
Fasching 2011 Abstract Berlin2011Fasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract Berlin.

O2k-Manual and protocols

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