O2k-Publications: O2k-Protocol

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O2k-Publications: O2k-Protocol

O2k-Publications in the MiPMap

»O2k-Publications: Topics

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 YearReferenceOrganismTissue;cell
MiPNet12.23 FibreRespiration2019-01-10
O2k-Protocols contents
Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle.
HorseSkeletal muscle
MiPNet20.14 AmplexRed H2O2-production2018-11-26
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed.
MouseHeart
MiPNet22.16 DatLab 7 Innovations: DL-Protocols2018-11-26
O2k-Protocols
DatLab 7.3 Innovations: DL-Protocols
MiPNet23.12 DL-Protocols help2018-11-26
O2k-Protocols
DatLab Protocols: Help DatLab 7.
MiPNet18.06 Amplex-Yeast2018-11-25
O2k-Protocols
O2k-FluoRespirometry: Amplex UltraRed using freeze-dried baker’s yeast.
Saccharomyces cerevisiae
Fungi
MiPNet12.01 Suppl T-issue2018-11-18
O2k-Protocols contents
MitoPathways at the Q-junction: mouse skeletal muscle fibres.
MouseSkeletal muscle
MiPNet20.13 Safranin mt-membranepotential2018-09-25
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.
MouseNervous system
MiPNet21.16 DatLab 7 Innovations2018-07-12
About Oroboros
DatLab 7: innovations.
MiPNet21.06 SUIT RP2018-06-25
O2k-Protocols
SUIT reference protocol for OXPHOS analysis by high-resolution respirometry.
MiPNet21.17 BloodCellsIsolation2018-04-09
O2k-Protocols
O2k-Protocols: Isolation of blood cells for HRR.
HumanBlood cells
Lymphocyte
Platelet
Doerrier 2018 Methods Mol Biol2018Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70.Human
Mouse
Rat
Saccharomyces cerevisiae
Heart
Skeletal muscle
Endothelial;epithelial;mesothelial cell
Blood cells
HEK
Platelet
Komlodi 2018 Methods Mol Biol2018Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55.Human
Mouse
Skeletal muscle
HEK
Komlodi 2018 J Bioenerg Biomembr2018Komlódi T, Geibl FF, Sassani M, Ambrus A, Tretter L (2018) Membrane potential and delta pH dependency of reverse electron transport-associated hydrogen peroxide production in brain and heart mitochondria. J Bioenerg Biomembr 10.1007/s10863-018-9766-8.Guinea pigHeart
Nervous system
MiPNet15.09. Yeast: HRR Reference Assay2017-02-05
O2k-Protocols
A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast.
Saccharomyces cerevisiae
Fungi
Lemieux 2017 Sci Rep2017Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840, DOI:10.1038/s41598-017-02789-8.MouseHeart
MiPNet08.09 CellRespiration2016-08-11
O2k-Procedures contents
High-resolution respirometry and coupling control protocol with intact cells: ROUTINE, LEAK, ET-pathway, ROX.
HumanBlood cells
Lymphocyte
MiPNet17.05 O2k-Fluo LED2-Module2016-08-09
O2k-Manual
O2k-Fluo LED2-Module.
MiPNet19.18E O2 flux analysis - DatLab 62016-08-08
O2k-Manual
Oxygen flux analysis: DatLab real-time.
MiPNet10.04 CellRespiration2016-08-08
O2k-Protocols
An experiment with high-resolution respirometry: coupling control in cell respiration.
MouseBlood cells
MiPNet21.07 MitoFit DatLab PT2016-07-20
O2k-Protocols
MitoFit DatLab proficiency test.
Human
Schoepf 2016 FEBS J2016Schöpf B, Schäfer G, Weber A, Talasz H, Eder IE, Klocker H, Gnaiger E (2016) Oxidative phosphorylation and mitochondrial function differ between human prostate tissue and cultured cells. FEBS J 283:2181-96.HumanEndothelial;epithelial;mesothelial cell
Genital
Other cell lines
Fibroblast
Doerrier 2016 Mitochondrion2016Doerrier C, García JA, Volt H, Díaz-Casado ME, Luna-Sánchez M, Fernández-Gil B, Escames G, López LC, Acuña-Castroviejo D (2016) Permeabilized myocardial fibers as model to detect mitochondrial dysfunction during sepsis and melatonin effects without disruption of mitochondrial network. Mitochondrion 27:56-63.MouseHeart
MiPNet17.03 Shredder vs Fibres2015-06-18
O2k-Protocols
Mitochondrial respiration in permeabilized fibres versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
FishesHeart
Liver
MiPNet18.05 Amplex-Mouse-heart2015-06-16
O2k-Protocols
O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate.
MouseHeart
MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver2015-06-16
O2k-Protocols
Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver.
MouseHeart
Nervous system
Liver
MiPNet17.16 O2k-Spectrophotometry2015-06-16
O2k-Protocols
O2k-Spectrophotometry - A MitoCom Project.
Gnaiger 2015 Scand J Med Sci Sports2015Gnaiger E, Boushel R, Søndergaard H, Munch-Andersen T, Damsgaard R, Hagen C, Díez-Sánchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet JAL, Christiansen M, Helge JW, Saltin B (2015) Mitochondrial coupling and capacity of oxidative phosphorylation in skeletal muscle of Inuit and caucasians in the arctic winter. Scand J Med Sci Sports 25 (Suppl 4):126–34.HumanSkeletal muscle
Makrecka-Kuka 2015 Biomolecules2015
O2k-Protocols contents
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38.
Human
Mouse
Heart
Nervous system
HEK
Krumschnabel 2015 Methods Mol Biol2015
O2k-Protocols contents
Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61.
MouseNervous system
MiPNet12.11 MitoRespiration2014-02-20
O2k-Protocols
Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.
Artemia
Crustaceans
Krumschnabel 2014 Methods Enzymol2014
O2k-Protocols contents
Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.
MouseNervous system
Van Bergen 2014 Mitochondrion2014Van Bergen NJ, Blake RE, Crowston JG, Trounce IA (2014) Oxidative phosphorylation measurement in cell lines and tissues. Mitochondrion 15:24-33.Human
Mouse
Nervous system
Blood cells
Lymphocyte
Gnaiger 2014 MitoPathways2014
O2k-Protocols
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp.
Human
Mouse
Heart
Skeletal muscle
Fibroblast
Magnesium Green2013Chinopoulos C (2013) Use of Magnesium Green 5N hexapotassium salt to measure ADP-ATP exchange rates. Mitochondr Physiol Network 2013-02-14.
Pesta 2012 Methods Mol Biol2012
O2k-Protocols
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58.
HumanFibroblast
HEK
HUVEC
Other cell lines
Skeletal muscle
Votion 2012 PLoS One2012Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7:e34890.HorseSkeletal muscle
MiPNet12.17 BovineHeartMito2011-12-11
O2k-Protocols contents
Mitochondrial respiration with frozen bovine heart mitochondria: diagnostic tests.
BovinesHeart
MiPNet15.08 TPP electrode2011-12-11
O2k-Protocols
Ion selective electrode for TPP+ and high-resolution respirometry.
MiPNet08.12 IOC222011-12-11
O2k-Protocols
NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.
RatLiver
Lemieux 2011 Int J Biochem Cell Biol2011Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729–38.HumanHeart
MiPNet14.05 TPP-mtMembranePotential2009-01
O2k-Protocols contents
TPP+ and mt-membrane potential.
MouseNervous system
Gnaiger 2008 POS2008
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.
MouseBlood cells
Boushel 2007 Diabetologia2007Boushel RC, Gnaiger E, Schjerling P, Skovbro M, Kraunsoee R, Dela F (2007) Patients with Type 2 diabetes have normal mitochondrial function in skeletal muscle. Diabetologia 50:790-6.HumanSkeletal muscle
Huetter 2004 Biochem J2004Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28.HumanFibroblast
Gnaiger 2000 Life in the Cold2000
O2k-Protocols contents
Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42.
RatHeart
MiPNet04.05 Titration-Injection1999-01
O2k-Protocols contents
From step titration to ramp injection: Uncoupling by FCCP with TIP.
HumanEndothelial;epithelial;mesothelial cell
HUVEC
Steinlechner-Maran 1996 Am J Physiol Cell Physiol1996Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61.HumanEndothelial;epithelial;mesothelial cell
HUVEC
O2k-Publications in the MiPMap

O2k-Publications: O2k-Protocol- Abstracts

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 YearReferenceOrganismTissue;cell
Bastos Sant'Anna Silva 2018 TRANSMIT Bertinoro di Romagna IT2018Cellular succinate transport and mitochondrial respiratory function in prostate cancer.HumanHEK
Genital
Bastos Sant'Anna Silva 2018 MiPschool Tromso C42018
Ana Bastos Sant'Anna
Role of succinate in prostate cancer cells: uptake and mitochondrial respiratory function.
HumanGenital
Chang 2018 Life Sciences Meeting 2018 Innsbruck AT2018pH dependence of mitochondrial respiration and H2O2 production in oral cancer cells – a pilot study.HumanHEK
Kidney
Sobotka 2018 MiP20182018
Ondrej Sobotka
Measurement of ROS production under hypoxia and unexpected methodological pitfalls of Amplex UltraRed assay.
Mouse
Saccharomyces cerevisiae
Heart
Nervous system
Sobotka 2017 MiPschool Obergurgl2017
Ondrej Sobotka
Reference protocols for ROS production measurements.
Doerrier 2016 Abstract MitoFit Science Camp 20162016Development of a SUIT reference protocol for OXPHOS analysis by high-resolution respirometry.Human
Mouse
Heart
Blood cells
HEK
Platelet
Volani 2016 Abstract Mito Xmas Meeting Innsbruck2016Effects of systemic iron perturbations on mitochondrial activity and on cellular metabolism in vivo.MouseLiver
Laner 2015 Abstract MiPschool Greenville 20152015Comparative mitochondrial physiology: OXPHOS and ET capacity in permeabilized fibres of canine superathletes.DogSkeletal muscle
Sumbalova 2015 Abstract MiP20152015High-resolution measurement of mitochondrial membrane potential and respiration – comparison of potentiometric and fluorometric methods.MouseNervous system
Sumbalova 2014 Abstract MiP20142014Optimization of malate concentration for high-resolution respirometry: mitochondria from rat liver and brain.RatNervous system
Liver