O2k signals and output

From Bioblast
Jump to: navigation, search
Bioblasts - Richard Altmann and MiPArt by Odra Noel
Bioblast         MitoPedia         Terms and abbreviations         Concepts and methods         Oroboros O2k         MiP and biochemistry         Preprints and history



MitoPedia

O2k signals and output

Description

Three electronic channel types are available in the O2k-MultiSensor system. All channels are available twofold (dual-data), for O2k-Chambers A (left) and B (right), based on numerical signals sent at a fixed data sampling time interval (default: 2 s; range 0.2 s to >10 s).


Reference: O2k-Catalogue: O2k-MultiSensor


MitoPedia O2k and high-resolution respirometry: O2k hardware, DatLab 


O2k signals

up to O2k Series G
O2k Series H
1. O2 channel with electric current (ampere [A]) as the primary signal, which is converted to a voltage (volt [V]) the raw signal. The O2k-FluoRespirometer supports not only the O2 channel (O2 plug), but also the channel types 2 and 3 for the add-on modules.
2a. Fluo channel: The Fluo sensor has electric current (ampere [A]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. Therefore, the O2k-Fluo LED2-Module is connected to the Amp plug (up to O2k-Series G) or the Smart Fluo-Sensors are connected to the separate Fluo plug (O2k-Series H upwards).
2b. Amp channel: The amperometric channel of the O2k (Amp plug), has electric current (ampere [A]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. The Amp channel is used with the O2k-NO Amp-Module. Up to O2k-Series G, the Amp plug is also used with the O2k-Fluo LED2-Module. Electronically, 2a and 2b are the same channel.
3. pX channel with electric potential (volt [V]) as the primary and raw signal (BNC pX plug for the ion sensitive electrode, ISE; and separate plug for the pX reference electrode, Ref).


O2k-Innovation: additional channel types are available in the O2k-Innovation:

4. Dual-fluo channel.
5. Spectrophotometric channel.


System channels, for instrumental control and monitoring experimental conditions for both O2k-Chamber A and B (single-data). The numberical signals are sent at the same fixed data sampling time interval:
6a. Block temperature: Measured block temperature equivalent to the experimental temperature in the O2k-Chambers at steady state [°C].
6b. Peltier power (system channel): Relative heating or cooling power of the Peltier system.
6c. Barometric pressure (system channel): Measured barometric pressure [kPa].
6d. Ambient temperature (system channel): Measured temperature of room air (O2k-Series G upwards) [°C].
6e. External temperature (system channel): Temperature measured with an independently placed thermistor (O2k-Series G upwards) [°C].


Events carry information on a time point during the experiment, a short event name (text), and a longer string of comments (text):
7. Events: Automatic events (e.g. from the TIP2k) and manually triggered events.


O2k output

The 'output' of a channel is the finally important information, which may be summarized in a data base.


Calibrated signals and derived plots: Three types of output can be derived from the signal of the channels supported by the O2k and displayed as separate plots:
I: Concentration. Examples: O2; Ca2+; concentration is amount of substance per volume [1 µM = 1 µmol·dm-3 = 1 nmol·ml-1].
II: Flow, flux, rate. Examples: O2 consumption; H2O2 production; volume-specific flux is the negative or positive time derivative of concentration over time, frequently multiplied by 1,000 [pmol·s-1·ml-1]. Volume-specific flux normalized for the cell density [106·ml-1] is flow [pmol·s-1·ml-1]. Volume-specific flux normalized for the mass density of the sample in the O2k-Chamber [mg·ml-1] is mass-specific flux [pmol·s-1·mg-1]. Flux in any respiratory state normalized for flux in a defined reference state is a rate [dimensionless] or flux control ratio.
III: Force, potential. Examples: TMRM, Safranin and Rhodamine 123 for mt-membrane potential; redox potential from redox states of cytochromes or NADH .



O2k-Concept
Output types for the O2k-MultiSensor modules
1. O2 channel - all O2k configurations: Output type I and II.
2a. Fluo (Amp) channel - O2k-FluoRespirometer and O2k-Fluo LED2-Module: The focus of the output depends on the specific fluorophore. Output type I, II, or III, or any combination may relate to the primary level of interest.
2b. Amp channel - O2k-NO Amp-Module: Output type I and II.
3a. pX channel - O2k-TPP+ ISE-Module: Output type III.
3b. pX channel - O2k-pH ISE-Module: Output type I and II.


Additional output types for the O2k-Innovation
4. Single-fluo channel - O2k-Innovation: Plots of one selected wavelength can be selected for recording. Output type I(1), II(1), III(1) depends on the fluorophore.
5. Dual-fluo channel - O2k-Innovation: Plots of two selected wavelengths can be selected for simultaneous recording. Output type I(2), II(2), III(2) depends on the fluorophore.
6. Spectrophotometric channel - O2k-Innovation: Output type IV (three plots for redox states of cytochromes aa3, b, and c).
IV: Redox states.


V: Pre-calibrated signals for system channels.


VI: Events.