Orrico 2018 Free Radic Biol Med

Orrico F, Möller MN, Cassina A, Denicola A, Thomson L (2018) Kinetic and stoichiometric constraints determine the pathway of catalase; glutathione peroxidase; hydrogen peroxide; peroxiredoxin; reaction kinetic; reaction rate; red blood cells consumption by red blood cells. Free Radic Biol Med 121:231-9.

» PMID: 29753074»

Orrico F, Moeller MN, Cassina A, Denicola A, Thomson L (2018) Free Radic Biol Med

Abstract: Red blood cells (RBC) are considered as a circulating sink of H₂O₂, but a significant debate remains over the role of the different intraerythocyte peroxidases. Herein we examined the kinetic of decomposition of exogenous H₂O₂ by human RBC at different cell densities, using fluorescent and oxymetric methods, contrasting the results against a mathematical model. Fluorescent measurements as well as oxygen production experiments showed that catalase was responsible for most of the decomposition of H₂O₂ at cell densities suitable for both experimental settings (0.1-10 × 10¹⁰ cell L^-1), since sodium azide but not N-ethylmaleimide (NEM) inhibited H₂O₂ consumption. Oxygen production decreased at high cell densities until none was detected above 1.1 × 10¹² cell L^-1, being recovered after inhibition of the thiol dependent systems by NEM. This result underlined that the consumption of H₂O₂ by catalase prevail at RBC densities regularly used for research, while the thiol dependent systems predominate when the cell density increases, approaching the normal number in blood (5 × 10¹² cell L^-1). The mathematical model successfully reproduced experimental results and at low cell number it showed a time sequence involving Prx as the first line of defense, followed by catalase, with a minor role by Gpx. The turning points were given by the total consumption of reduced Prx in first place and reduced GSH after that. However, Prx alone was able to account for the added Catalase; Glutathione peroxidase; Hydrogen peroxide; Peroxiredoxin; Reaction kinetic; Reaction rate; Red blood cells (50 µM) at physiological RBC density, calling attention to the importance of cell density in defining the pathway of H₂O₂ consumption and offering an explanation to current apparently conflicting results in the literature. • Keywords: Catalase, Glutathione peroxidase, Hydrogen peroxide, Peroxiredoxin, Reaction kinetic, Reaction rate, Red blood cells • Bioblast editor: Kandolf G • O2k-Network Lab: UY Montevideo Radi R

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Labels: MiParea: Respiration 

Tissue;cell: Blood cells 

Regulation: Oxygen kinetics 

HRR: Oxygraph-2k 

Labels, 2018-07, O2k-brief