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Racker 1967 J Biol Chem

From Bioblast
Publications in the MiPMap
Racker E, Horstman LL (1967) Partial resolution of the enzymes catalyzing oxidative phosphorylation XIII. Structure and function of submitochondrial particles completely resolved with respect to coupling factor 1. J Biol Chem 242:2547-51.

Β» PMID: 4290709 Open Access

Racker E, Horstman LL (1967) J Biol Chem

Abstract:

  1. A Mg2+-dependent adenosine triphosphatase was solubilized and purified from bakers' yeast mitochondria. The enzyme resembled mitochondrial ATPase from beef heart with respect to substrate specificity, cold lability, and other physical properties.
  2. An antiserum against the purified yeast enzyme inhibited the ATPase activity of the soluble enzyme as well as ATPase and oxidative phosphorylation in submitochondrial yeast particles. Mitochondrial ATPase from beef heart or from Neurospora crassa was not inhibited by the antiserum.
  3. Submitochondrial beef heart particles devoid of endogenous ATPase could bind the purified yeast enzyme without changing its immunological specificity. The ATPase activity of the resultant "hybrid" particles, like that of beef heart particles, was strongly inhibited by low levels of rutamycin. In contrast, submitochondrial particles from yeast were much less sensitive to this inhibitor.
  4. The yeast enzyme stimulated oxidative phosphorylation in beef heart particles which were deficient in, but not devoid of, endogenous ATPase. The stimulation was dependent on the presence of beef heart coupling factor 1 (F1) in these particles and was unaffected by the antiserum against the yeast enzyme. Antiserum against beef heart F1 strongly inhibited phosphorylation. These results suggest that yeast F1, in contrast to beef heart F1, does not significantly participate in phosphate transfer reactions when it functions as a coupling factor in beef heart particles. Rather, it is proposed that the stimulation by yeast F1 is due to an effect on the membrane structure.

β€’ Keywords: Oxidative phosphorylation, Beef heart submitochondrial particles, Coupling factor 1, Yeast ATPase


Labels: MiParea: Respiration 


Organism: Bovines 

Preparation: SMP  Enzyme: Complex V;ATP synthase, Marker enzyme  Regulation: ADP 



Made history