Substrate-uncoupler-inhibitor titration

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Substrate-uncoupler-inhibitor titration

Description

Mitochondrial Substrate-uncoupler-inhibitor titration (SUIT) protocols are used with mitochondrial preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental assay.

Abbreviation: SUIT

Reference: Gnaiger 2014 MitoPathways, MiPNet09.12, MitoPedia: SUIT


MitoPedia concepts: MiP concept, SUIT concept 


MitoPedia methods: Respirometry 


MitoPedia topics: Inhibitor, Substrate and metabolite, Uncoupler 

Contributed by Gnaiger E 2010-09-18, edited 2016-01-24.

Pesta and Gnaiger 2012

SUIT protocols

SUIT protocols have been developed for the first time on the basis of high-resolution respirometry, since their application depends critically on long-term stability of the polarographic oxygen sensor (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: Gnaiger_2000_MitoInTheCold; MiR06) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature.
Attempts are in progress to develop a database of SUIT protocols


References