Truu 2017 Abstract IOC122

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Bioenergetics of colorectal cancer.

Link: Mitochondr Physiol Network 22.01

Truu L, Chekulayev V, Klepinin A, Ounpuu L, Tepp K, Puurand M, Koit A, Shevchuk I, Kaambre T (2017)

Event: IOC122

Bioenergetics is a fast growing field in cancer research, where many promising outcomes could provide targeted cancer treatment. Energy metabolism specific literature is characterized by many contradictions, concluding that cancer cells metabolize their increased glucose uptake via glycolysis rather than more energy efficient oxidative phosphorylation (OXPHOS). Furthermore, the majority of these conclusions are the outcome of only in vitro studies on cell culture models, without taking into consideration the factors arising from the tumor microenvironment giving significant effects in vivo. We have conducted quantitative cellular respiration analysis on normal colon tissue, colorectal cancer (HCC) clinical tissue samples and CaCo-2 cell cultures. Our results show that HCC is not a fully glycolytic tumor and OXPHOS system might be the main source of ATP. Comparing healthy colon, HCC tissue and CaCo-2 cells, we found elevated rates of maximal ADP-activated respiration and greater activity of respiratory complex (C) II over CI in both HCC and CaCo-2 cells, whereas the opposite result in healthy tissue was present. These results indicate that the bioenergetic profile of Caco-2 cells corresponds generally to HCC tissue. Further research is in progress to generate a full cancer development model consisting of cell cultures, clinical polyps and malignant versus healthy tissue samples.


Bioblast editor: Kandolf G O2k-Network Lab: EE Tallinn Kaambre T


Labels: MiParea: Respiration  Pathology: Cancer 


Tissue;cell: Endothelial;epithelial;mesothelial cell 


Coupling state: OXPHOS  Pathway: N, S  HRR: Oxygraph-2k 


Affiliations

Truu L(1), Chekulayev V(1), Klepinin A(1), Ounpuu L(1), Tepp K(1), Puurand M(1), Koit A(1), Shevchuk I (1), Kaambre T(1,2)
  1. Lab Bioenergetics, Nat Inst Chemical Physics Biophysics
  2. Tallinn Univ, School Natural Sci Health
Tallinn, Estonia. - laura.truu@gmail.com