MiPNet22.03 IOC119 Innsbruck AT
 Innsbruck AT, 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic IOC119. |
OROBOROS (2017-02-27) Mitochondr Physiol Network
Abstract:
MitoFit Coaching Days - Basic. Innsbruck, Austria; 2017 Feb 28-Mar 03.
• O2k-Network Lab: AT_Innsbruck_OROBOROS, AT_Innsbruck_MitoFit
Programme
| Tuesday 28th | Topic | Room |
| 17:00 - 18:00 | Welcome - common aims | MiPArt |
| Wednesday 1st | Topic | Room |
| 09:00 | Mitochondrial and cell research | MiPArt |
| 10:00 | OroboPOS service and O2k instrumental setup - overview with videoclips | MiPArt |
| 10:30 | Hands-on: OroboPOS service and O2k instrumental setup | MiPArt |
| 12:00 | Lunch break | |
| 13:00 | Instrumental quality control 1: oxygen calibration | MiPArt |
| 14:00 | Instrumental quality control 2: Instrumental O2 background | MiPArt |
| 15:00 | Coffee | |
| 15:30 | Hands-on: Instrumental quality control 1 and 2. DatLab analysis. | MitoFit ORO Lab |
| 14:30-18:00 | Hands-on: Instrumental quality control 1 and 2. | MitoFit ORO Lab - MiPArt |
| Thursday 2nd | Topic | Room |
| 09:00 | Demo: Instrumental quality control 1. Introduction to SUIT protocols RP1&RP2 | MiPArt |
| 10:00 | Coffee | |
| 10:30 | O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. | MiPArt |
| 12:00 | Lunch break | |
| 13:00 | Introduction to DatLab analysis | MiPArt |
| 15:00 | Coffee | |
| 15:30-18:00 | Individual DatLab analysis + summary | MiPArt |
| Friday 3rd | Topic | Room |
| 09:00 | SUIT protocols | MiPArt |
| 10:30 | Coffee | |
| 11:00 | Q&A session on HRR and OXPHOS analysis: Design of experimental protocols | MiPArt |
| 12:00 | Lunch break | |
| 13:00 | O2k-Core and O2k-Multisensor | MiPArt |
| 14:00 | DatLab analysis | MiPArt |
| 15:00 | Coffee | |
| 15:30 | DatLab analysis | MiPArt |
| 16:15 | The Bioblast wiki, O2k-Network and MITOEAGLE - feedback & conclusions | MiPArt |
| 17:30 | Farewell activity | MiPArt |
General information
- The MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the O2k-Core, instrumental assembly and technical service, the DatLab software, oxygen sensor calibration, oxygen flux analysis of respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
- » Information on travel and venue: IOC Innsbruck.
Registration
- Fully booked.
- Maximum number of 6 participants.
Tutors
Participants
| Participant | Institution | |
|---|---|---|
| Anja Bufe | AT Innsbruck OROBOROS: OROBOROS INSTRUMENTS, Innsbruck (AT) | |
| Eugenia Carvalho | US AR Little Rock Boersheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US) | |
| Matthew Cotter | US AR Little Rock Boersheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US) | |
| Ivone Leong | SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE) | |
| Yue Hugh Guan | CN Shanghai Yi X: East China University of Science and Technology, Shanghai (CN) | |
| Vasco Sequeira Oliveira | NL Amsterdam Wuest RC: VU University Medical Center, Amsterdam (NL) | |
| Anna-Maria Wintler | SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE) | |
| Evelyn Zacharewicz | NL Maastricht Schrauwen P: Department of Human Biology and Movement Sciences, Maastricht University (NL) |
Contact
- OROBOROS INSTRUMENTS
- high-resolution respirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
Project support
- Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
IOC recommended reading
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Labels:
ORO, IOC, 2017, MitoFit, Next
