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Run DL-Protocol/Set O2 limit

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Revision as of 15:15, 13 November 2016 by Gnaiger Erich (talk | contribs) (Created page with "{{MitoPedia |abbr=n.a. |description='''Protocols''' can be selected in DatLab (DL7.1) which define the sequence of events] and [[Marks in DatLab |marks. Ins...")
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{{MitoPedia |abbr=n.a. |description=Protocols can be selected in DatLab (DL7.1) which define the sequence of [[Events in DatLab |events] and marks. Instrumental protocols are used for calibrations and instrumental quality control, typically without experimental smaple in the incubation medium. Substrate-uncoupler-inhibitor titration protocols (see MitoPedia: SUIT) proceed stepwise to activate a sequence of coupling control states and pathway control states. A specific SUIT protocol can be assigned to O2k-chamber A or B or both. The Titration-Injection-microPump TIP2k can be programmed to control automatically TIP2k-protocols. Standard protocols are provided. These can be edited and saved under 'Lab protocols' or under the User logged into DatLab. }}




MitoPedia O2k and high-resolution respirometry: DatLab 



Protocol editor

The sequence of Events and Marks can be edited with names, definitions, concentrations and titration volumes. After a protocol is edited and saved under a defined name, the protocol has to be selected and assigned to one or both O2k-chambers.
A. Instrumental protocols: calibration and instrumental O2 background test
B. SUIT protocols: linked to titration solutions
C. TIP2k-protocols.


Protocol driver

After selecting a protocol, the Protocol driver indicates for the selected O2k-chamber, which events and marks have been set (green), and which event is to be set next, with the corresponding mark.