2-Deoxyglucose

From Bioblast


high-resolution terminology - matching measurements at high-resolution


2-Deoxyglucose

Description

2-Deoxyglucose, also known as 2-deoxy-D-glucose is a glucose derivative that has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase.

Abbreviation: 2-DG

Reference: Proton_flux, O2k-pH_ISE-Module

Application in HRR

2-DG: 2-deoxy-D-glucose (C6H1205); Sigma D6134, 1 g or 5 g, store at 2-8 Β°C; M = 164.16 gΒ·mol-1
Preparation of 2 M stock solution (dissolved in H2O):
  1. weigh 1 g of 2-deoxy-D-glucose
  2. dissolve with 3.0458 mL H2O in a 10 mL-glass beaker
Β»O2k manual titrations: MiPNet09.12 O2k-Titrations
  • Titration volume: 25 Β΅L using a 50 Β΅L syringe
  • Final conc. in 2 mL O2k-chamber: 25 mM






MitoPedia topics: Inhibitor 

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