Agrimi 2014 Abstract IOC 2014-04 Schroecken

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Agrimi G, Mena MC, Izumi K, Pisano I, Germinario L, Fukuzaki H, Palmieri L, Blank LM, Kitagaki H (2014) Increased mitochondrial pyruvate dissimilation in sake yeast. Mitochondr Physiol Network 19.02.

Link:

Agrimi G, Mena MC, Izumi K, Pisano I, Germinario L, Fukuzaki H, Palmieri L, Blank LM, Kitagaki H (2014)

Event: MiPNet19.02 IOC88

Although the decrease of pyruvate secretion by brewer’s yeasts during fermentation has long been desired in the alcohol beverage industry, rather little is known about the regulation of pyruvate accumulation. In this study, we have characterized a previously developed a pyruvate undersecreting sake yeast obtained by isolating a strain (TCR7) tolerant to ethyl α-transcyanocinnamate, an inhibitor of pyruvate transport into mitochondria. To obtain insights into pyruvate metabolism, we investigated the mitochondrial activity of TCR7 by oxigraphy and 13C-metabolic flux analysis during aerobic growth . While mitochondrial pyruvate oxidation was higher, glycerol production was decreased in TCR7 compared to the reference. These results indicate that mitochondrial activity is elevated in the TCR7 strain with the consequence of decreased pyruvate accumulation. Surprisingly mitochondrial activity is much higher in the sake yeast compared to CEN.PK 113-7D, the reference strain in metabolic engineering. When shifted from aerobic to anaerobic conditions, sake yeast retains a branched mitochondrial structure for a longer time than laboratory strains. The regulation of mitochondrial activity can become a completely novel approach to manipulate metabolic profile during fermentation of brewer’s yeasts.

Abstract Agrimi G Graphical.jpg


O2k-Network Lab: IT Bari Palmieri L


Labels: MiParea: Respiration, mt-Membrane 


Organism: Saccharomyces cerevisiae 

Preparation: Intact cells, Isolated mitochondria  Enzyme: Inner mt-membrane transporter, TCA cycle and matrix dehydrogenases  Regulation: Aerobic glycolysis, Flux control, Substrate 


HRR: Oxygraph-2k 

Biotechnology 

Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona 4, 70125 Bari, Italy

Department of Environmental Sciences, Faculty of Agriculture, Saga University, 1 Honjo-cho, Saga 840-8502, Japan

Institute of Applied Microbiology - iAMB, ABBt – Aachen Biology and Biotechnology Department, RWTH Aachen University, 52074 Aachen, Germany