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Andriamihaja 2018 Mol Metab

From Bioblast
Publications in the MiPMap
Andriamihaja M, Lan A, Beaumont M, Grauso M, Gotteland M, Pastene E, Cires MJ, Carrasco‑Pozo C, Tomé D, Blachier F (2018) Proanthocyanidin‑containing polyphenol extracts from fruits prevent the inhibitory effect of hydrogen sulfide on human colonocyte oxygen consumption. Mol Metab 12:39-47.

» PMID: 29706321

Andriamihaja M, Lan A, Beaumont M, Grauso M, Gotteland M, Pastene E, Cires MJ, Carrasco‑Pozo C, Tome D, Blachier F (2018) Mol Metab

Abstract: Hydrogen sulfide (H2S), a metabolic end product synthesized by the microbiota from L-cysteine, has been shown to act at low micromolar concentration as a mineral oxidative substrate in colonocytes while acting as an inhibitor of oxygen consumption at higher luminal concentrations (65 µM and above). From the previous works showing that polyphenols can bind volatile sulfur compounds, we hypothesized that different dietary proanthocyanidin-containing polyphenol (PACs) plant extracts might modulate the inhibitory effect of H2S on colonocyte respiration. Using the model of human HT-29 Glc-/+ cell colonocytes, we show here that pre-incubation of 65 µM of the H2S donor NaHS with the different polyphenol extracts markedly reduced the inhibitory effect of NaHS on colonocyte oxygen consumption. Our studies on HT-29 Glc-/+ cell respiration performed in the absence or the presence of PACs reveal rapid binding of H2S with the sulfide-oxidizing unit and slower binding of H2S to the cytochrome c oxidase (complex IV of the respiratory chain). Despite acute inhibition of colonocyte respiration, no measurable effect of NaHS on paracellular permeability was recorded after 24 h treatment using the Caco-2 colonocyte monolayer model. The results are discussed in the context of the binding of excessive bacterial metabolites by unabsorbed dietary compounds and of the capacity of colonocytes to adapt to changing luminal environment. Keywords: Colonocyte, Hydrogen sulfide, Oxygen consumption, Polyphenols, Proanthocyanidins Bioblast editor: Kandolf G

Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, Pharmacology;toxicology 

Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell, Other cell lines  Preparation: Intact cells 

Coupling state: OXPHOS 

HRR: Oxygraph-2k 

Labels, 2018-07