Belosludtsev 2019 Cells

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Belosludtsev KN, Talanov EY, Starinets VS, Agafonov AV, Dubinin MV, Belosludtseva NV (2019) Transport of Ca2+ and Ca2+-dependent permeability transition in rat liver mitochondria under the streptozotocin-induced type I Diabetes. Cells 8:E1014.

» PMID: 31480399 Open Access

Belosludtsev KN, Talanov EY, Starinets VS, Agafonov AV, Dubinin MV, Belosludtseva NV (2019) Cells

Abstract: Although diabetes mellitus is known to be a disease associated with mitochondrial dysfunction, not everything is clear about mitochondrial Ca2+ transport and Ca2+-induced permeability transition in diabetic cells. The objective of this work was to study the operation of MCU and Ca2+-dependent mitochondrial permeabilization in the liver cells of Sprague-Dawley rats under the streptozotocin-induced type I diabetes. It was shown that two weeks after the induction of diabetes, the rate of Ca2+ uptake by the mitochondria of diabetic animals increased ~1.4-fold. The expression of MCU and MICU1 subunits did not change, yet the quantity of dominant-negative MCUb channel subunits was almost twice as lower. The organelles also became more resistant to the induction of CsA-sensitive MPT pore and less resistant to the induction of CsA-insensitive palmitate/Ca2+-induced pore. The mitochondria of diabetic liver cells also showed changes in the lipid matrix of their membranes. The content of fatty acids in the membranes grew, and microviscosity of the lipid bilayer (assessed with laurdan) increased. At the same time, lipid peroxidation (assessed by the production of malonic dialdehyde) was stimulated. The paper discusses the consequences of the diabetes-related changes in mitochondria in the context of cell physiology.

Keywords: Ca2+ uniporter, MPT pore, Calcium, Diabetes mellitus, Lipid pore, Mitochondria, Palmitic acid Bioblast editor: Plangger M

Labels: MiParea: Respiration, mt-Membrane  Pathology: Diabetes  Stress:Permeability transition  Organism: Rat  Tissue;cell: Liver  Preparation: Isolated mitochondria 

Coupling state: LEAK  Pathway:HRR: Oxygraph-2k 

Labels, 2019-11