Bernhardt 2015 Sci Rep

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Bernhardt D, Müller M, Reichert AS, Osiewacz HD (2015) Simultaneous impairment of mitochondrial fission and fusion reduces mitophagy and shortens replicative lifespan. Sci Rep 5:7885.

» PMID:25601284 Open Access

Bernhardt D, Mueller M, Reichert AS, Osiewacz HD (2015) Sci Rep

Abstract: Aging of biological systems is accompanied by degeneration of mitochondrial functions. Different pathways are active to counteract the processes which lead to mitochondrial dysfunction. Mitochondrial dynamics, the fission and fusion of mitochondria, is one of these quality control pathways. Mitophagy, the controlled degradation of mitochondria, is another one. Here we show that these pathways are linked. A double deletion mutant of Saccharomyces cerevisiae in which two essential components of the fission and fusion machinery, Dnm1 and Mgm1, are simultaneously ablated, contain wild-type like filamentous mitochondria, but are characterized by impaired respiration, an increased sensitivity to different stressors, increased mitochondrial protein carbonylation, and a decrease in mitophagy and replicative lifespan. These data show that a balanced mitochondrial dynamics and not a filamentous mitochondrial morphotype per se is the key for a long lifespan and demonstrate a cross-talk between two different mitochondrial quality control pathways.

Keywords: Mitophagy, Senescence

O2k-Network Lab: DE Frankfurt Osiewacz HD

Labels: MiParea: Respiration, mt-Structure;fission;fusion, mtDNA;mt-genetics, Genetic knockout;overexpression  Pathology: Aging;senescence 

Organism: Saccharomyces cerevisiae 

Preparation: Intact cells 

Coupling state: ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k