Bettink 2019 Mitochondrion

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Publications in the MiPMap
Bettink MAW, Harms FA, Dollee N, Specht PAC, Raat NJH, Schoonderwoerd GC, Mik EG (2019) Non-invasive versus ex vivo measurement of mitochondrial function in an endotoxemia model in rat: toward monitoring of mitochondrial therapy. Mitochondrion 50:149-57.

» PMID: 31770610 Open Access

Bettink MAW, Harms FA, Dollee N, Specht PAC, Raat NJH, Schoonderwoerd GC, Mik EG (2019) Mitochondrion

Abstract: Mitochondrial function has been predominantly measured ex vivo. Due to isolation and preservation procedures ex vivo measurements might misrepresent in vivo mitochondrial conditions. Direct measurement of in vivo mitochondrial oxygen tension (mitoPO2) and oxygen disappearance rate (ODR) with the protoporphyrin IX-triplet state lifetime technique (PpIX-TSLT) might increase our understanding of mitochondrial dysfunction in the pathophysiology of acute disease. LPS administration decreased mitochondrial respiration (ODR) in vivo but did not alter mitochondrial function as assessed with ex vivo techniques (high resolution respirometry and specific complex determinations). PpIX-TSLT measures in vivo mitoPO2 and ODR and can be applied non-invasively at the skin.

Copyright © 2019. Published by Elsevier B.V.

Keywords: 5-aminolevulinic acid, Endotoxemia, Lipopolysaccharides, Mitochondria, Succinic Acid Bioblast editor: Plangger M O2k-Network Lab: NL Rotterdam Schoonderwoerd KGC


Labels: MiParea: Respiration, Instruments;methods 


Organism: Rat  Tissue;cell: Skeletal muscle  Preparation: Homogenate 


Coupling state: LEAK, OXPHOS, ET  Pathway: N, S  HRR: Oxygraph-2k 

Labels, 2019-12