Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Bioblast alert 2011

From Bioblast

Bioblast alert 2011

Bioblast wiki
Bioblast alert

back to "Bioblast alert" main page

Bioblast alert 2011(01): 2011-11-11

  • A key question on the separation of qualitative versus quantitative change of mitochondrial function is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?
Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.
  • ET capacity plays a key role in such analyses, which cannot be achieved quantitatively without careful uncoupler titrations.
  • Basal respiration - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology.


Bioblast alert 2011(02): 2011-11-17

  • Heart failure and mitochondrial dysfunction. Increased OXPHOS capacity after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.
Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738. --> Discussion: Complementary results on the PGC-1a pathway.
  • Tissue storage on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with SUIT protocols.
Evaluate an example of low-resolution terminology: Uncoupling was studied in stressed cells by measurement of coupled respiration, evaluation of uncoupled respiration after inhibition with oligomycin, and FCCP titration to quantify uncoupled respiration.


Bioblast alert 2011(03): 2011-11-24

  • Fluorometry combined with high-resolution respirometry: Light guide inserted into the chamber through a black PEEK stopper[1], or optical probe operated through the O2k-glass window[2].

Bioblast alert 2011(04): 2011-12-24

Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58.