Canto 2015 Curr Protoc Mouse Biol
|Cantó C, Garcia-Roves PM (2015) High-resolution respirometry for mitochondrial characterization of ex vivo mouse tissues. Curr Protoc Mouse Biol 5:135-53.|
Abstract: This article describes methodologies to examine mitochondrial respiration in fresh preparations of mouse tissues, including skeletal muscle, heart, liver, white and brown adipose tissue, and brain. Reference values and tips to maximize experimental efficiencies are also provided. Finally, correction methods and complementary techniques to properly interpret the results are presented and contrasted.
• Keywords: Bioenergetics, Mitochondrial physiology, Respiratory complexes
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse Tissue;cell: Heart, Skeletal muscle, Nervous system, Liver, Fat Preparation: Permeabilized tissue, Homogenate
Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS HRR: Oxygraph-2k