Cervinkova 2008 Physiol Res

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Cervinková Z, Rauchová H, Kriváková P, Drahota Z (2008) Inhibition of palmityl carnitine oxidation in rat liver mitochondria by tert-butyl hydroperoxide. Physiol Res 57:133-6.

» PMID: 17465699 Open Access

Cervinkova Z, Rauchova H, Krivakova P, Drahota Z (2008) Physiol Res

Abstract: Mitochondria as an energy generating cell device are very sensitive to oxidative damage. Our previous findings obtained in hepatocytes demonstrated that Complex I of the respiratory chain is more sensitive to oxidative damage than other respiratory chain complexes. We present additional data on isolated mitochondria showing that palmityl carnitine oxidation is strongly depressed at a low (200 microM) tert-butyl hydroperoxide (tBHP) concentration, while oxidation of the flavoprotein-dependent substrate-succinate is not affected and neither is ATP synthesis inhibited by tBHP. In the presence of tBHP, the respiratory control index for palmityl carnitine oxidation is strongly depressed, but when succinate is oxidized the respiratory control index remains unaffected. Our findings thus indicate that flavoprotein-dependent substrates could be an important nutritional factor for the regeneration process in the necrotic liver damaged by oxidative stress.

Keywords: Tert-butyl hydroperoxide (tBHP), Palmityl carnitine oxidation

O2k-Network Lab: CZ Hradec Kralove Cervinkova Z


Labels: MiParea: Respiration 

Stress:Oxidative stress;RONS 

Tissue;cell: Liver  Preparation: Isolated mitochondria  Enzyme: Complex I, Complex II;succinate dehydrogenase  Regulation: Fatty acid 

Pathway: F, N, S  HRR: Oxygraph-2k