Ceusters 2013 Res Vet Sci

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Ceusters Justine D, Mouithys-Mickalad AA, Franck TJ, Deby-Dupont GP, Derochette S, Serteyn DA (2013) Effect of different kinds of anoxia/reoxygenation on the mitochondrial function and the free radicals production of cultured primary equine skeletal myoblasts. Res Vet Sci 95:870-8.

» PMID: 24099743

Ceusters Justine D, Mouithys-Mickalad AA, Franck TJ, Deby-Dupont GP, Derochette S, Serteyn DA (2013) Res Vet Sci

Abstract: Horses are outstanding athletes, performing in many different disciplines involving different kinds of efforts and metabolic responses. Depending on exercise intensity, their skeletal muscle oxygenation decreases, and the reperfusion at cessation of the exercise can cause excessive production of free radicals. This study on cultured primary equine myoblasts investigated the effect of different kinds of anoxia/reoxygenation (A/R) on routine respiration, mitochondrial complex I specific activity and free radicals production. Our data revealed that short cycles of A/R caused a decrease of all the parameters, opposite to what a single long period of anoxia did. A preconditioning-like effect could explain our first pattern of results whereas mild uncoupling could be more appropriate for the second one. Anyway, it seems that mitochondrial complex I could play a major role in the regulation of the balance between metabolic and antioxidant protection of the muscular function of athletic horses.

Keywords: Cultured primary equine skeletal myoblasts, Anoxia/reoxygenation, Mitochondria, Reactive oxygen species

O2k-Network Lab: BE Liege Votion DM


Labels: MiParea: Respiration 

Stress:Ischemia-reperfusion, Oxidative stress;RONS  Organism: Horse  Tissue;cell: Skeletal muscle  Preparation: Intact cells 


Coupling state: ROUTINE  Pathway:HRR: Oxygraph-2k