Chamber volume

high-resolution terminology - matching measurements at high-resolution

Chamber volume

Description

The chamber volume of the O2k is 2.0 mL or 0.5 mL of aqueous medium with or without sample, excluding the volume of the stirrer and the volume of the capillary of the stopper (see: Cell count and normalization in HRR). A modular extension of the O2k, the O2k-sV-Module, was specifically developed to perform high-resolution respirometry with reduced amounts of biological sample, and all components necessary for the smaller operation volume of 0.5 mL.

Reference: MiPNet22.11 O2k-FluoRespirometer manual

Keywords

»Bioblast links: Chamber volume - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

» Volume

• Units: Liter [L]; 1 L = 1 dm³ = 10^-3 m³; 1 mL = 1 cm³; 1 µL = 1 mm³

» Oroboros chamber

» The experimental chamber in the instrumental Oroboros Chamber

» O2k-Chamber sV

» Calibration of Oroboros chamber volume

• On chamber volume

» Oroboros chamber

» Chamber volume

» Oroboros paradigm shift

» Cell count and normalization in HRR

» Open chamber

» Closed chamber

• Components of the experimental Oroboros chambers

» Stirrer-Bar\white PVDF\15x6 mm

» Stirrer-Bar sV\white PVDF\11.5x6.2 mm

» Stopper\black PEEK\conical Shaft\central Port

» Stopper sV\black PEEK\conical Shaft\central Port

» O-ring\Viton\12.5x1 mm

» O-ring sV\Viton\9.5x1 mm

» Volume-Calibration Ring

» Volume-Calibration Ring sV

» Stopper-Spacer

» Cover-Slip\black

» O2k-Chamber Holder

» O2k-Chamber Holder sV

» OroboPOS-Holder

» OroboPOS-Holder sV

• Maintencance

» Oroboros chamber cleaning and ISS

» Quality control: Oroboros chamber test

MitoPedia O2k and high-resolution respirometry: DatLab