Chao 2019 Am J Physiol Cell Physiol

From Bioblast
Jump to: navigation, search
Publications in the MiPMap
Chao T, Gomez BI, Heard TC, Smith BW, Dubick MA, Burmeister DM (2019) Burn-induced reductions in mitochondrial abundance and efficiency are more pronounced with small volumes of colloids in swine. Am J Physiol Cell Physiol 317:C1229-38.

» PMID: 31532719

Chao T, Gomez BI, Heard TC, Smith BW, Dubick MA, Burmeister DM (2019) Am J Physiol Cell Physiol

Abstract: Severe burn injury results in systemic disruption of metabolic regulations and impaired cardiac function. Restoration of hemodynamic homeostasis utilizing intravenous (IV) fluids is critical for acute care of the burn victim. However, the effects of burns and resuscitation on cardiomyocyte mitochondria are currently unknown. The purpose of this study is to determine cardiac mitochondrial function in a swine burn model with subsequent resuscitation using either crystalloids or colloids. Anesthetized Yorkshire swine (n=23) sustained 40% total body surface area (TBSA) burns and received IV crystalloids (n=11) or colloids (n=12) after recovery from anesthesia. Non-burned swine served as control (n=9). After euthanasia at 48h, heart tissues were harvested, permeabilized, and analyzed by high-resolution respirometry. Citrate synthase (CS) activity was measured, and Western blots were performed to quantify proteins associated with mitochondrial fusion (OPA1), fission (FIS1), and mitophagy (PINK1). There were no differences in State-2 respiration or maximal oxidative phosphorylation. Coupled Complex-1 respiration decreased, while uncoupled State-4O, and complex-II increased significantly due to burn injury, particularly in animals receiving colloids (p<0.05). CS activity and electron transfer coupling efficiency were significantly lower in burned animals, particularly with colloid treatment (p<0.05). Protein analysis revealed increased FIS1, but no differences in mitophagy in cardiac tissue from colloid-treated compared to crystalloid-treated swine. Taken together, severe burns alter mitochondrial respiration in heart tissue, which may be exacerbated by early IV resuscitation with colloids. Early IV burn resuscitation with colloids may require close hemodynamic observation. Mitochondrial stabilizing agents incorporated into resuscitation fluids may help the hemodynamic response to burn injury.

Keywords: Burn, Heart, Mitochondria, Resuscitation, Swine Bioblast editor: Plangger M

Labels: MiParea: Respiration, mt-Medicine 

Stress:Temperature  Organism: Pig  Tissue;cell: Heart  Preparation: Permeabilized tissue 

Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, S, NS  HRR: Oxygraph-2k 

Labels, 2019-10