Closed chamber

From Bioblast
Jump to: navigation, search

Oroboros Instruments

News         O2k-Products         O2k-Purchase         O2k-Open Support         O2k-Publications         O2k-Workshops and Events         O2k-Network         O2k-Feedback         NextGen-O2k



Closed chamber

O2k-Open Support
This information is part of O2k-Open Support.

Closed chamber means that the chamber and the capillary of the stopper is filled with liquid and no air space or bubble is present in the chamber. For closing the chamber without a bubble an excessive amount of liquid has to be present in the chamber (2.5 ml for 2 ml chamber). The conical shape of the standard stopper facilitates bubble-free closing of the chamber with stirrers switched on - the bubbles will escape through the capillary. If some bubble remains in the chamber, the capillary should be relieved by inserting long needle into the capillary. After closing the chamber the excessive liquid should be aspirated from the top of the stopper. The procedure of bubble-free closing closing a chamber with a multisensor stopper is described in the manual for ion selective electrodes MiPNet15.03.

See also: Open chamber