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Coupling-control state

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Coupling-control state

Description

Coupling-control states are defined in mitochondrial preparations (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as LEAK respiration, OXPHOS, and ET states, with corresponding respiration rates (L, P, E) in any electron-transfer-pathway state which is competent for electron transfer. These coupling states are induced by titration of ADP and uncouplers, and application of specific inhibitors of the phosphorylation pathway. In living cells, the coupling-control states are LEAK respiration, ROUTINE, and ET states of respiration with corresponding rates L, R, E, using membrane-permeable inhibitors of the phosphorylation system (e.g. oligomycin) and uncouplers (e.g. CCCP). Coupling-control protocols induce these coupling-control states sequentially at a constant electron-transfer-pathway state.

Abbreviation: CCS

Reference: BEC 2020.1, Gnaiger 2020 BEC MitoPathways, Gnaiger 2009 Int J Biochem Cell Biol

Coupling-control states: OXPHOS capacity ROUTINE respiration ET capacity LEAK respiration - ROX

Communicated by Gnaiger E (2011-02-25) last update 2020-11-11.

SUITbrowser question: Coupling control

Coupling-control protocols are SUIT protocols that allow to analyze coupling control in living cells or mitochondrial preparations.
Use the SUITbrowser to find the best protocol to answer this and other research questions.

References

Keywords


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1. Mitochondrial and cellular respiratory rates in coupling-control states

OXPHOS-coupled energy cycles. Source: The Blue Book
ยป Baseline state
Respiratory rate Defining relations Icon
OXPHOS capacity P = Pยด-Rox P.jpg mt-preparations
ROUTINE respiration R = Rยด-Rox R.jpg living cells
ET capacity E = Eยด-Rox E.jpg ยป Level flow
ยป Noncoupled respiration - Uncoupler
LEAK respiration L = Lยด-Rox L.jpg ยป Static head
ยป LEAK state with ATP
ยป LEAK state with oligomycin
ยป LEAK state without adenylates
Residual oxygen consumption Rox L = Lยด-Rox ROX.jpg
  • Chance and Williams nomenclature: respiratory states
ยป State 1 โ€”ยป State 2 โ€”ยป State 3 โ€”ยป State 4 โ€”ยป State 5

2. Flux control ratios related to coupling in mt-preparations and living cells

ยป Flux control ratio
ยป Coupling-control ratio
ยป Coupling-control protocol
FCR Definition Icon
L/P coupling-control ratio L/P L/P coupling-control ratio ยป Respiratory acceptor control ratio, RCR = P/L
L/R coupling-control ratio L/R L/R coupling-control ratio
L/E coupling-control ratio L/E L/E coupling-control ratio ยป Uncoupling-control ratio, UCR = E/L (ambiguous)
P/E control ratio P/E P/E control ratio
R/E control ratio R/E R/E control ratio ยป Uncoupling-control ratio, UCR = E/L
net P/E control ratio (P-L)/E net P/E control ratio
net R/E control ratio (R-L)/E net R/E control ratio

3. Net, excess, and reserve capacities of respiration

Respiratory net rate Definition Icon
P-L net OXPHOS capacity P-L P-L net OXPHOS capacity
R-L net ROUTINE capacity R-L R-L net ROUTINE capacity
E-L net ET capacity E-L E-L net ET capacity
E-P excess capacity E-P E-P excess capacity
E-R reserve capacity E-R E-R reserve capacity

4. Flux control efficiencies related to coupling-control ratios

ยป Flux control efficiency jZ-Y
ยป Background state
ยป Reference state
ยป Metabolic control variable
Coupling-control efficiency Definition Icon Canonical term
P-L control efficiency jP-L = (P-L)/P = 1-L/P P-L control efficiency P-L OXPHOS-flux control efficiency
R-L control efficiency jR-L = (R-L)/R = 1-L/R R-L control efficiency R-L ROUTINE-flux control efficiency
E-L coupling efficiency jE-L = (E-L)/E = 1-L/E E-L coupling efficiency E-L ET-coupling efficiency ยป Biochemical coupling efficiency
E-P control efficiency jE-P = (E-P)/E = 1-P/E E-P control efficiency E-P ET-excess flux control efficiency
E-R control efficiency jE-R = (E-R)/E = 1-R/E E-R control efficiency E-R ET-reserve flux control efficiency

5. General

ยป Basal respiration
ยป Cell ergometry
ยป Dyscoupled respiration
ยป Dyscoupling
ยป Electron leak
ยป Electron-transfer-pathway state
ยป Hyphenation
ยป Oxidative phosphorylation
ยป Oxygen flow
ยป Oxygen flux
ยป Permeabilized cells
ยป Phosphorylation system
ยป Proton leak
ยป Proton slip
ยป Respiratory state
ยป Uncoupling



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Specific
ยป Artefacts by single dose uncoupling
ยป ATP synthase
ยป CCCP
ยป Coupling-control protocol
ยป DNP
ยป Dyscoupled respiration
ยป FCCP
ยป Is respiration uncoupled - noncoupled - dyscoupled?
ยป Noncoupled respiration: Discussion
ยป Uncoupler
ยป Uncoupled respiration - see ยป Noncoupled respiration
ยป Uncoupling proteins
ยป Uncoupling protein 1
ยป Uncoupler titrations - Optimum uncoupler concentration
Respiratory states and control ratios
ยป Biochemical coupling efficiency
ยป Coupling-control state
ยป Electron-transfer-pathway state
ยป Electron-transfer pathway
E.jpg ET capacity
ยป E-L coupling efficiency
ยป Flux control efficiency
ยป Flux control ratio
ยป LEAK-control ratio
ยป LEAK respiration
ยป Noncoupled respiration
ยป OXPHOS
ยป OXPHOS capacity; ยป State 3
ยป OXPHOS-control ratio, P/E ratio
ยป Respiratory acceptor control ratio
ยป ROUTINE-control ratio
ยป ROUTINE respiration
ยป ROUTINE state
ยป State 3u
ยป State 4
ยป Uncoupling-control ratio UCR
General (alphabetical order)
ยป Adenine nucleotide translocase
ยป Adenylates
ยป Electron transfer pathway
ยป Mitochondrial preparations
ยป mt-membrane potential
ยป Oxygen flux
ยป Phosphorylation system
ยป Proton leak
ยป Proton slip
ยป TIP2k
Other keyword lists
ยป Template:Keywords: Force and membrane potential



MitoPedia concepts: MiP concept, Respiratory state, SUIT concept, SUIT state, Recommended 


MitoPedia methods: Respirometry 


MitoPedia topics: EAGLE