Crescenzo 2019 Mol Neurobiol

From Bioblast
Publications in the MiPMap
Crescenzo R, Spagnuolo MS, Cancelliere R, Iannotta L, Mazzoli A, Gatto C, Iossa S, Cigliano L (2019) Effect of initial aging and high-fat/high-fructose diet on mitochondrial bioenergetics and oxidative status in rat brain. Mol Neurobiol 56:7651-63.

Β» PMID: 31089964 Β»O2k-brief

Crescenzo Raffaella, Spagnuolo Maria Stefania, Cancelliere Rosa, Iannotta Lucia, Mazzoli Arianna, Gatto Cristina, Iossa Susanna, Cigliano Luisa (2019) Mol Neurobiol

Abstract: Middle age is an early stage of the aging process, during which the consumption of diets rich in saturated fats and/or simple sugars might influence brain function, but only few data are available on this issue. We therefore investigated the impact of a diet rich in saturated fat and fructose (HFF) on mitochondrial physiology in hippocampus and frontal cortex of middle-aged rats (1 year old), by including a group of adult rats (90 days) as a "negative control," lacking the putative effect of aging. Middle-aged rats were fed HFF or control diet for 4 weeks. Mitochondrial function was analyzed by high-resolution respirometry and by assessing the amount of respiratory complexes. Markers of oxidative balance, as well as the protein content of uncoupling protein 2 (UCP2), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1Ξ±), and peroxisome proliferator-activated receptor alpha (PPARΞ±), were also assessed. A decrease in the activity of complex I was detected in both brain areas of middle-aged rats. In hippocampus, mitochondrial respiratory capacity and complex IV content decreased with age and increased with HFF diet. Higher protein oxidative damage, decreased antioxidant defenses, and increased UCP2 and PGC-1Ξ± content were found in hippocampus of middle-aged rats. HFF feeding induced a significant reduction in the amount of UCP2, PGC-1Ξ±, and PPARΞ±, together with higher protein oxidative damage, in both brain areas. Overall, our results point to middle age as a condition of early brain aging for mitochondrial function, with hippocampus being an area more susceptible to metabolic impairment than frontal cortex. β€’ Keywords: Cortex, Fructose, Hippocampus, Middle age, Mitochondria, Western diet β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: IT Naples Iossa S

Labels: MiParea: Respiration, Exercise physiology;nutrition;life style  Pathology: Aging;senescence 

Organism: Rat  Tissue;cell: Nervous system  Preparation: Homogenate  Enzyme: Uncoupling protein 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS, ROX  HRR: Oxygraph-2k 

Labels, 2019-05, Agenda2020, O2k-brief 

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