Cunha 2017 Int J Biol Macromol

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Cunha de Padua MM, Suter Correia Cadena SM, de Oliveira Petkowicz CL, Martinez GR, Rodrigues Noleto G (2017) Galactomannan from Schizolobium amazonicum seed and its sulfated derivatives impair metabolism in HepG2 cells. Int J Biol Macromol 101:64-473.

» PMID: 28347788

Cunha de Padua MM, Suter Correia Cadena SM, de Oliveira Petkowicz CL, Martinez GR, Rodrigues Noleto G (2017) Int J Biol Macromol

Abstract: This study evaluated the effects of native galactomannan from Schizolobium amazonicum seeds and its sulfated forms on certain metabolic parameters of HepG2 cells. Aqueous extraction from S. amazonicum seeds furnished galactomannan with 3.2:1 Man:Gal ratio (SAGM) and molar mass of 4.34×105g/mol. The SAGM fraction was subjected to sulfation using chlorosulfonic acid to obtain SAGMS1 and SAGMS2 with DS of 0.4 and 0.6, respectively. Cytotoxicity of SAGM, SAGMS1, and SAGMS2 was evaluated in human hepatocellular carcinoma cells (HepG2). After 72h, SAGM decreased the viability of HepG2 cells by 50% at 250μg/mL, while SAGMS1 reduced it by 30% at the same concentration. SAGM, SAGMS1, and SAGMS2 promoted a reduction in oxygen consumption and an increase in lactate production in non-permeabilized HepG2 cells after 72h of treatment. These results suggest that SAGM, SAGMS1, and SAGMS2 could be recognized by HepG2 cells and might trigger alterations that impair its survival. These effects could be implicated in the modification of the oxidative phosphorylation process in HepG2 cells and activation of the glycolytic pathway.

Copyright © 2017 Elsevier B.V. All rights reserved.

Keywords: Galactomannans, HepG2 cells, Sulfation Bioblast editor: Kandolf G


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, Pharmacology;toxicology  Pathology: Cancer 

Organism: Human  Tissue;cell: Liver  Preparation: Intact cells 


Coupling state: LEAK, ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k 

2017-06