Dawson 2020 J Exp Biol
|Dawson NJ, Millet C, Selman C, Metcalfe NB (2020) Measurement of mitochondrial respiration in permeabilized fish gills. J Exp Biol 223:jeb216762.|
Abstract: Physiological investigations of fish gills have traditionally been centered on the two principal functions of the gills, gas exchange and ion regulation. Mitochondrion-rich cells (MRCs) are primarily found within the gill filaments of fish, and are thought to proliferate in order to increase the ionoregulatory capacity of the gill in response to environmentally-induced osmotic challenges. However, surprisingly little attention has been paid to the metabolic function of mitochondria within fish gills. Here we describe and validate a simple protocol for the permeabilization of fish gills and subsequent measurement of mitochondrial respiration rates in vitro Our protocol requires only small tissue samples (8 mg), it exploits the natural structure of fish gills, does not require mechanical separation of the gill tissue (so is relatively quick to perform), and yields accurate and highly reproducible measurements of respiration rates. It offers great potential for the study of mitochondrial function in gills over a wide range of fish sizes and species.
© 2020. Published by The Company of Biologists Ltd.
• Keywords: Electron transport chain, Fish, Gills, Mitochondria rich cells, Oxidative phosphorylation, Oxygen consumption, Oxygraph • Bioblast editor: Plangger M • O2k-Network Lab: UK Glasgow Metcalfe NB
Labels: MiParea: Respiration
Organism: Fishes Tissue;cell: Lung;gill Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS Pathway: N, CIV, NS, ROX HRR: Oxygraph-2k