Dejean 2002 Biochem Biophys Res Comm

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Dejean L, Beauvoit B, Bunoust O, Guerin B, Rigoulet M (2002) Activation of Ras cascade increases the mitochondrial enzyme content of respiratory competent yeast. Biochem Biophys Res Comm 293:1383-8.

» PMID: 12054668

Dejean L, Beauvoit B, Bunoust O, Guerin B, Rigoulet M (2002) Biochem Biophys Res Commun

Abstract: We investigated the effects of genetic and physiological modulations of the cAMP–protein kinase A pathway on mitochondrial biogenesis of yeast cells grown on lactate. Yeast mutants with over-activated Ras/adenylate cyclase pathway (i.e., Ras2val19, ira1Δira2Δ) or with a constitutive downstream activation of protein kinases A (i.e., bcyΔ) showed an increase in the mitochondrial enzyme content. In contrast, loss of Ras activity (i.e., Ras2 mutant) resulted in a slight decrease. The treatment by cAMP of a responsive mutant increased the oxidative phosphorylation capacity of cells and increased the transcript level of nuclear genes encoding for mitochondrial proteins. In contrast, the transcript level of mitochondrial DNA genes was unchanged. It is concluded that the Ras/cAMP/protein kinase A pathway is part of the regulatory circuit controlling biogenesis of the oxidative phosphorylation complexes in yeast cells.

Keywords: cAMP, Saccharomyces cerevisiae, Ras mutants, Mitochondria, Cytochromes, Respiration

O2k-Network Lab: FR Pessac Diolez P, FR Bordeaux Devin A

Labels: MiParea: Respiration, Genetic knockout;overexpression 

Organism: Saccharomyces cerevisiae, Fungi 

Preparation: Intact cells 

Coupling state: OXPHOS 

HRR: Oxygraph-2k