Deline 2021 Biochim Biophys Acta Bioenerg
|Deline ML, Grashei M, van Heijster FHA, Schilling F, Straub J, Fromme T (2021) Adenylate kinase derived ATP shapes respiration and calcium storage of isolated mitochondria. Biochim Biophys Acta Bioenerg 1862:148409.|
Abstract: The ratio of ADP and ATP is a natural indicator of cellular bioenergetic state and thus a prominent analyte in metabolism research. Beyond adenylate interconversion via oxidative phosphorylation and ATPase activities, ADP and ATP act as steric regulators of enzymes, e.g. cytochrome C oxidase, and are major factors in mitochondrial calcium storage potential. Consideration of all routes of adenylate conversion is critical to successfully predict their abundance in an experimental system and to correctly interpret many aspects of mitochondrial function.
We showcase here how adenylate kinases elicit considerable impact on the outcome of a variety of mitochondrial assays through their drastic manipulation of the adenylate profile. Parameters affected include cytochrome c oxidase activity, P/O ratio, and mitochondrial calcium dynamics. Study of the latter revealed that the presence of ATP is required for mitochondrial calcium to be shaped into a particularly dense form of mitochondrial amorphous calcium phosphate. • Keywords: Adenylate kinase, Amorphous calcium phosphate, Calcium capacity, Cytochrome c oxidase, Mitochondria, P/O ratio • Bioblast editor: Reiswig R
Labels: MiParea: Respiration
Organism: Mouse Tissue;cell: Liver Preparation: Isolated mitochondria
Regulation: ADP, ATP Coupling state: LEAK, OXPHOS Pathway: S HRR: Oxygraph-2k, O2k-Fluorometer, Ca