Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Dlaskova 2010 Biochim Biophys Acta

From Bioblast
Publications in the MiPMap
Dlasková A, Clarke KJ, Porter RK (2010) The role of UCP 1 in production of reactive oxygen species by mitochondria isolated from brown adipose tissue. Biochim Biophys Acta 1797:1470-6.

» PMID: 20416274 Open Access; PDF

Dlaskova A, Clarke KJ, Porter RK (2010) Biochim Biophys Acta

Abstract: We provide evidence that ablation or inhibition of, uncoupling protein 1 increases the rate of reactive oxygen containing species production by mitochondria from brown adipose tissue, no matter what electron transport chain substrate is used (succinate, glycerol-3-phosphate or pyruvate/malate). Consistent with these data are our observations that (a) the mitochondrial membrane potential is maximal when uncoupling protein 1 is ablated or inhibited and (b) oxygen consumption rates in mitochondria from uncoupling protein 1 knock-out mice, are significantly lower than those from wild-type mice, but equivalent to those from wild-type mice in the presence of GDP. In summary, we show that uncoupling protein 1 can affect reactive oxygen containing species production by isolated mitochondria from brown adipose tissue. Keywords: Mitochondria, Reactive oxygen species, Brown adipose tissue, Uncoupling protein 1

O2k-Network Lab: IE Dublin Porter RK


Labels: MiParea: Respiration, Genetic knockout;overexpression 

Stress:Oxidative stress;RONS  Organism: Mouse  Tissue;cell: Fat  Preparation: Isolated mitochondria  Enzyme: Uncoupling protein 

Coupling state: OXPHOS 

HRR: Oxygraph-2k