Doerrier 2016a Abstract Mito Xmas Meeting Innsbruck
K-Regio MitoFit and MitoEAGLE – towards a global data bank on mitochondrial physiology.
Burtscher M, Dikova V, Doerrier C, Drinnan M, Garcia-Souza LF, Gnaiger E, Krumschnabel G, Laner V, Plattner C, Sumbalova Z (2016)
Event: Mito Xmas Meeting 2016 Innsbruck AT
A lack of physical activity associates with decreased mitochondrial capacity and is a major cause underlying metabolic dysregulation and preventable diseases in modern societies. In contrast, an active lifestyle supports enhanced mitochondrial capacities and reduces the risk of degenerative diseases. Despite of this well-known relation between health and mitochondrial function, there is no regimented, quantitative system, or database organised to routinely test, compare and monitor mitochondrial capacities within individuals or populations. Using high-resolution respirometry, the MitoFit and MitoEAGLE initiatives will develop novel lab standards and diagnostic methods for monitoring of a mitochondrial fitness score. To this end, SOPs will be worked out regarding sample preparation, respiratory evaluation and data documentation. Fresh and cryopreserved cells obtained noninvasively from blood samples will serve as models, the latter allowing samples to be collected for later analysis, thereby broadening the scope for respirometric investigations. This approach will then be expanded to all sorts of human tissues and cells of interest and assess aspects relating to Evolution, Age, Gender, Lifestyle and Environment (EAGLE) as essential background conditions characterizing the individual patient, subject, study group, and/or species. The huge scope of this endeavour requires an international network of laboratories capable of generating the necessary number of consistent data to address the complexity of EAGLE. Coping with the mass of the expected data necessitates a dedicated MitoEAGLE knowledge management network developing harmonization protocols towards generating a rigorously monitored data repository on mitochondrial respiratory function. The resulting MitoEAGLE data management system will enable to interrelate results of a large number of studies, to interpret pathological phenotypes, and to set results into the multidimensional context of EAGLE.
• O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck Oroboros, AT Innsbruck Burtscher M, SK Bratislava Sumbalova Z
Labels: MiParea: Respiration, Comparative MiP;environmental MiP, Gender, Exercise physiology;nutrition;life style, mt-Awareness Pathology: Aging;senescence
Organism: Human Tissue;cell: Skeletal muscle, Blood cells
- Burtscher M(1), Dikova V(2), Doerrier C(3), Drinnan M(4), Garcia-Souza LF(4), Gnaiger E(2,3), Krumschnabel G(3), Laner V(3), Plattner C(5), Sumbalova Z(3)
- Inst Sport Science, University Innsbruck, Austria
- Dept Visceral, Transplant and Thoracic Surgery, Daniel Swarovski Research Laboratory, Medical University Innsbruck, Austria
- Oroboros Instruments, Innsbruck, Austria
- Regional Medical Physics Department, Freeman Hospital, Newcastle upon Tyne, United Kingdom
- Inst Bioinformatics, Medical University Innsbruck, Austria
- K-Regio project K-Regio MitoFit. European Union Framework Programme Horizon 2020 COST Action CA15203 MitoEAGLE