Doerrier 2022 MitoFit NADH

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Publications in the MiPMap
Doerrier C, Cardoso LHD, Gnaiger E (2022) Simultaneous measurement of oxygen consumption and NAD(P)H/NAD(P)+-redox state in isolated mitochondria. (in prep)

Abstract: Keywords: NADH fluorescence, NAD-pool, N-junction, NAD-redox state, high-resolution respirometry HRR, isolated mitochondria, mouse liver mitochondria, SUIT protocols, N-pathway Bioblast editor: Doerrier C O2k-Network Lab: AT Innsbruck Oroboros


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Bradshaw PC (2019) Cytoplasmic and Mitochondrial NADPH-Coupled Redox Systems in the Regulation of Aging. Nutrients 11:504.2019PMID:30818813 Open Access
Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. 2021.1Bioenerg Commun 2021.1. BEC pdf published online 2021-06-30

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Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Mol Biol 1782:31-70. PMID: 29850993 »O2k-brief
Endogenous quinones sustain NADH oxidation by Complex I during anoxia, supporting substrate-level phosphorylation in mouse liver mitochondria.
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Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. 2020.2Bioenerg Commun 2020.2:112 pp. Open Access pdf published online 2020-12-30

Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. 2020.1Bioenerg Commun 2020.1. Open Access pdf published online 2020-05-20

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O2k-Protocols contents
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Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55.2018PMID:29850998
Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. 2021.3Bioenerg Commun 2021.3. Open Access pdf published online 2021-11-11

Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. 2021.4Bioenerg Commun 2021.4. Open Access pdf published online 2021-12-21

Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Enzymol 542:163-81. PMID: 24862266 »O2k-brief
O2k-Protocols contents
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Selected media and chemicals for respirometry with mitochondrial preparations.2016-08-30Bioblast pdf  »Versions
Stincone A, Prigione A, Cramer T, Wamelink MMC, Campbell K, Cheung E, Olin-Sandoval V, Grüning NM, Krüger A, Tauqeer Alam M, Keller MA, Breitenbach M, Brindle KM, Rabinowitz JD, Ralser M (2014) The return of metabolism: biochemistry and physiology of the pentose phosphate pathway. Biol Rev Camb Philos Soc 90:927-63.2014/ PMID: 25243985 Open Access
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Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523.2017PMID: 29091971 Open Access »O2k-brief
Xiao W, Wang RS, Handy DE, Loscalzo J (2018) NAD(H) and NADP(H) redox couples and cellular energy metabolism. Antioxid Redox Signal 28:251–72.2018PMID: 28648096 Open Access
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Mitochondrial Respiration Medium - MiR05-Kit.
2022-03-03Bioblast pdf » Versions
NextGen-O2k: NADH-Module manual
2022-05-17PDF.jpg » Versions
Laboratory protocol: Isolation of rat liver mitochondria.
2010-08-03Bioblast pdf »Versions
de Jager TL, Cockrell AE, Du Plessis SS (2017) Ultraviolet Light Induced Generation of Reactive Oxygen Species. Adv Exp Med Biol 996:15-23.2017PMID: 29124687



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Mitochondrial NADH and respiration

» Nicotinamide adenine dinucleotide
» NADH electron transfer-pathway state
» N-junction
» Complex I
» Rotenone
» Amytal
» Piericidin

Mitochondrial NADH production

» Tricarboxylic acid cycle
» Fatty acid oxidation


» NextGen-O2k

Labels: MiParea: Respiration, Instruments;methods 

Organism: Mouse  Tissue;cell: Liver  Preparation: Isolated mitochondria 

Regulation: Redox state  Coupling state: LEAK, OXPHOS, ET  Pathway:HRR: NextGen-O2k