Dutra 2016 Acta Trop

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Dutra FL, Oliveira MM, Santos RS, Silva WS, Alviano DS, Vieira DP, Lopes AH (2016) Effects of linalool and eugenol on the survival of Leishmania (L.) infantum chagasi within macrophages. Acta Trop 164:69-76.

» PMID: 27591136

Dutra FL, Oliveira MM, Santos RS, Silva WS, Alviano DS, Vieira DP, Lopes AH (2016) Acta Trop

Abstract: The most commonly used drugs against visceral leishmaniasis are based on pentavalent antimonial com-pounds, which have played a fundamental role in therapy for over 70 years. However, the treatment is painful and has severe toxic side effects that can be fatal. Antimonial resistance is spreading and reaching alarming proportions. Linalool and eugenol have been shown to kill Leishmania (L.) amazonensis and Trypanosoma cruzi at low doses. In the present study, we demonstrate the effects of linalool and eugenol, components of essential oils, on Leishmania (L.) infantum chagasi, one of the causative agents of visceralleishmaniasis. We compared the effects of those compounds to the effects of glucantime, a positive control. In L. infantum chagasi killing assays, the LD50 for eugenol was 220 μg/ml, and that for linalool was 550 μg/ml. L. infantum chagasi was added to cultures of peritoneal mouse macrophages for four hours prior to drug treatment. Eugenol and linalool significantly decreased the number of parasites within themacrophages. Eugenol and linalool enhanced the activities of the L. infantum chagasi protein kinases PKA and PKC. Linalool also decreased L. infantum chagasi oxygen consumption. In conclusion, both linalool andeugenol promoted a decrease in the proliferation and viability of L. infantum chagasi. These effects were more pronounced during the interaction between the parasites and peritoneal mouse macrophages. Keywords: Eugenol, Linalool, Leishmania, Survival, Infectivity Bioblast editor: Kandolf G

Labels: MiParea: Respiration, Pharmacology;toxicology 

Organism: Protists 

Preparation: Intact organism, Intact cells 

Coupling state: ROUTINE  Pathway: ROX  HRR: Oxygraph-2k