I am often asked by reviewers to discuss the effects of pentobarbitol euthansia on mithochondrial function.
Takaki 1997 JJP: This paper has been helpful in this discussion. (edit by Staples JF)
Reference: Takaki M, Nakahara H, Kawatani Y, Utsumi K, Suga H (1997) No suppression of respiratory function of mitochondria isolated from the hearts of anesthetized rats with high-dose pentobarbital sodium. Jpn J Physiol 47:87–92
Organism: Rat Tissue;cell: Heart Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.