Flis 2018 Oxid Med Cell Longev

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Publications in the MiPMap
Flis DJ, Dzik K, Kaczor JJ, Halon-Golabek M, Antosiewicz J, Wieckowski MR, Ziolkowski W (2018) Swim training modulates skeletal muscle energy metabolism, oxidative stress, and mitochondrial cholesterol content in amyotrophic lateral sclerosis mice. Oxid Med Cell Longev 5940748.

» Open Access

Flis DJ, Dzik K, Kaczor JJ, Halon-Golabek M, Antosiewicz J, Wieckowski MR, Ziolkowski W (2018) Oxid Med Cell Longev

Abstract: Recently, in terms of amyotrophic lateral sclerosis (ALS), much attention has been paid to the cell structures formed by the mitochondria and the endoplasmic reticulum membranes (MAMs) that are involved in the regulation of Ca2+ signaling, mitochondrial bioenergetics, apoptosis, and oxidative stress. We assumed that remodeling of these structures via swim training may accompany the prolongation of the ALS lifespan. In the present study, we used transgenic mice with the G93A hmSOD1 gene mutation. We examined muscle energy metabolism, oxidative stress parameters, and markers of MAMs (Caveolin-1 protein level and cholesterol content in crude mitochondrial fraction) in groups of mice divided according to disease progression and training status. The progression of ALS was related to the lowering of Caveolin-1 protein levels and the accumulation of cholesterol in a crude mitochondrial fraction. These changes were associated with aerobic and anaerobic energy metabolism dysfunction and higher oxidative stress. Our data indicated that swim training prolonged the lifespan of ALS mice with accompanying changes in MAM components. Swim training also maintained mitochondrial function and lowered oxidative stress. These data suggest that modification of MAMs might play a crucial role in the exercise-induced deceleration of ALS development.

Bioblast editor: Kandolf G O2k-Network Lab: PL Gdansk Kaczor JJ, PL Warsaw Szewczyk A

Labels: MiParea: Respiration, Exercise physiology;nutrition;life style  Pathology: Neurodegenerative 

Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Isolated mitochondria 

Coupling state: LEAK, OXPHOS  Pathway:HRR: Oxygraph-2k 

Labels, 2018-05