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R-L net ROUTINE capacity

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(Redirected from Free ROUTINE activity)


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R-L net ROUTINE capacity

Description

R-L net ROUTINE capacity The R-L net ROUTINE capacity is ROUTINE respiration corrected for LEAK respiration. R-L is the respiratory capacity available for phosphorylation of ADP to ATP. Oxygen consumption in the ROUTINE state of respiration measured in living cells, therefore, is partitioned into the R-L net ROUTINE capacity, strictly coupled to phosphorylation Pยป, and nonphosphorylating LEAK respiration, LR, compensating for proton leaks, slip and cation cycling: R = R-L+LR. It is frequently assumed that LEAK respiration L, as measured in the LEAK state, overestimates the LEAK component of respiration, LR, as measured in the ROUTINE state, particularly if the protonmotive force is not adjusted to equivalent levels in L and LR. However, if the LEAK component increases with enzyme turnover during R, the low enzyme turnover during L may counteract the effect of the higher pmF.

Abbreviation: R-L

Reference: Gnaiger 2020 BEC MitoPathways

Communicated by Gnaiger E (2014-09-21) last update 2020-11-11

Keywords

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1. Mitochondrial and cellular respiratory rates in coupling-control states

OXPHOS-coupled energy cycles. Source: The Blue Book
ยป Baseline state
Respiratory rate Defining relations Icon
OXPHOS capacity P = Pยด-Rox P.jpg mt-preparations
ROUTINE respiration R = Rยด-Rox R.jpg living cells
ET capacity E = Eยด-Rox E.jpg ยป Level flow
ยป Noncoupled respiration - Uncoupler
LEAK respiration L = Lยด-Rox L.jpg ยป Static head
ยป LEAK state with ATP
ยป LEAK state with oligomycin
ยป LEAK state without adenylates
Residual oxygen consumption Rox L = Lยด-Rox ROX.jpg
  • Chance and Williams nomenclature: respiratory states
ยป State 1 โ€”ยป State 2 โ€”ยป State 3 โ€”ยป State 4 โ€”ยป State 5

2. Flux control ratios related to coupling in mt-preparations and living cells

ยป Flux control ratio
ยป Coupling-control ratio
ยป Coupling-control protocol
FCR Definition Icon
L/P coupling-control ratio L/P L/P coupling-control ratio ยป Respiratory acceptor control ratio, RCR = P/L
L/R coupling-control ratio L/R L/R coupling-control ratio
L/E coupling-control ratio L/E L/E coupling-control ratio ยป Uncoupling-control ratio, UCR = E/L (ambiguous)
P/E control ratio P/E P/E control ratio
R/E control ratio R/E R/E control ratio ยป Uncoupling-control ratio, UCR = E/L
net P/E control ratio (P-L)/E net P/E control ratio
net R/E control ratio (R-L)/E net R/E control ratio

3. Net, excess, and reserve capacities of respiration

Respiratory net rate Definition Icon
P-L net OXPHOS capacity P-L P-L net OXPHOS capacity
R-L net ROUTINE capacity R-L R-L net ROUTINE capacity
E-L net ET capacity E-L E-L net ET capacity
E-P excess capacity E-P E-P excess capacity
E-R reserve capacity E-R E-R reserve capacity

4. Flux control efficiencies related to coupling-control ratios

ยป Flux control efficiency jZ-Y
ยป Background state
ยป Reference state
ยป Metabolic control variable
Coupling-control efficiency Definition Icon Canonical term
P-L control efficiency jP-L = (P-L)/P = 1-L/P P-L control efficiency P-L OXPHOS-flux control efficiency
R-L control efficiency jR-L = (R-L)/R = 1-L/R R-L control efficiency R-L ROUTINE-flux control efficiency
E-L coupling efficiency jE-L = (E-L)/E = 1-L/E E-L coupling efficiency E-L ET-coupling efficiency ยป Biochemical coupling efficiency
E-P control efficiency jE-P = (E-P)/E = 1-P/E E-P control efficiency E-P ET-excess flux control efficiency
E-R control efficiency jE-R = (E-R)/E = 1-R/E E-R control efficiency E-R ET-reserve flux control efficiency

5. General

ยป Basal respiration
ยป Cell ergometry
ยป Dyscoupled respiration
ยป Dyscoupling
ยป Electron leak
ยป Electron-transfer-pathway state
ยป Hyphenation
ยป Oxidative phosphorylation
ยป Oxygen flow
ยป Oxygen flux
ยป Permeabilized cells
ยป Phosphorylation system
ยป Proton leak
ยป Proton slip
ยป Respiratory state
ยป Uncoupling



MitoPedia concepts: Respiratory state, Recommended 


MitoPedia topics: EAGLE