Fromme 2021 Abstract Kalorimetrietage

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Fromme T (2021) Application of different respirometric assay systems to characterize the membrane permeabilization potency of saponins. Kalorimetrietage 2021.

Link: 24th Kalorimetrietage 2021 Braunschweig DE

Fromme Tobias (2021)

Event: 24th Kalorimetrietage 2021 Braunschweig DE

Saponins are a diverse class of secondary plant metabolites, some of which known for their ability to permeabilize biological membranes. We characterized the potency of several pure saponins to permeabilize plasma membrane and mitochondrial membranes of cultured, human cells for different metabolites. An initial screen made use of the advantages of a multiplate-format flux analyzer (Agilent Seahorse) while detailed validation exploited the strengths of a high-resolution respirometer (Oroboros O2k). This study highlights specific strengths and potential synergy of methodologically different cellular respirometry approaches.


Bioblast editor: Gnaiger E O2k-Network Lab: DE Freising Klingenspor M


Affiliation

Tobias Fromme
Chair of Molecular Nutritional Medicine, Technical University Munich (TUM) School of Life Sciences, Munich, Germany

Labels: MiParea: Respiration 



Preparation: Permeabilized cells 



HRR: Oxygraph-2k 

Saponin