GM-pathway control state

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Bioblasts - Richard Altmann and MiPArt by Odra Noel
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MitoPedia

GM-pathway control state

Description

GM

GM: Glutamate & Malate.

MitoPathway control state: NADH electron transfer-pathway state

The GM-pathway control state (glutamate-malate pathway control state) is established when glutamate&malate are added to isolated mitochondria, permeabilized cells and other mitochondrial preparations. Glutamate and transaminase are responsible for the metabolism of oxaloacetate, comparable to the metabolism with acetyl-CoA and citrate synthase.

Abbreviation: GM

Reference: Gnaiger 2020 BEC MitoPathways

More details
» NADH electron transfer-pathway state


GML

GM pathway in the LEAK state can be evaluated in the following SUIT protocols:

GMP

GM pathway in the OXPHOS state can be evaluated in the following SUIT protocols:
  • DL-Protocol for permeabilized cells (pce):

GME

GM pathway in the ET state can be evaluated in the following SUIT protocols:
  • DL-Protocol for permeabilized cells (pce):

Discussion

In human skeletal muscle mitochondria, respiration with GM in the presence of ADP (GMP) is identical or 10 % higher than with PM (PMP). These results on isolated mitochondria agree with permeabilized fibres, although there are reports on respiratory capacity for PMP being 16% to 25% higher than for GMP. In fibroblasts, GMP supports a higher respiratory flux than PMP.


MitoPedia concepts: SUIT state