Garedew 2005 Eur Shock Soc
|Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The Oroboros O2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna:107-11.|
Abstract: High-resolution respirometry with mitochondria, cells or biopsies is based on the unique features of the Oroboros O2k, combining instrumental design, electronics and task-specific DatLab software. Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump TIP2k is applied for kinetics as a modular component of the O2k. DatLab provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information.
Labels: MiParea: Respiration, Instruments;methods
Organism: Human Tissue;cell: Fibroblast Preparation: Intact cells
Regulation: Uncoupler Coupling state: OXPHOS
HRR: Oxygraph-2k, TIP2k