Gerna 2021 Int J Mol Sci

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Publications in the MiPMap
Gerna D, Arc E, Holzknecht M, Roach T, Jansen-Duerr P, Weiss AKH, Kranner I (2021) AtFAHD1a: a new player influencing seed longevity and dormancy in arabidopsis?. Int J Mol Sci 22:2997.

» PMID: 33804275 Open Access

Gerna Davide, Arc Erwann, Holzknecht Max, Roach Thomas, Jansen-Duerr Pidder, Weiss Alexander K H, Kranner Ilse (2021) Int J Mol Sci

Abstract: Fumarylacetoacetate hydrolase (FAH) proteins form a superfamily found in Archaea, Bacteria, and Eukaryota. However, few fumarylacetoacetate hydrolase domain (FAHD)-containing proteins have been studied in Metazoa and their role in plants remains elusive. Sequence alignments revealed high homology between two Arabidopsis thaliana FAHD-containing proteins and human FAHD1 (hFAHD1) implicated in mitochondrial dysfunction-associated senescence. Transcripts of the closest hFAHD1 orthologue in Arabidopsis (AtFAHD1a) peak during seed maturation drying, which influences seed longevity and dormancy. Here, a homology study was conducted to assess if AtFAHD1a contributes to seed longevity and vigour. We found that an A. thaliana T-DNA insertional line (Atfahd1a-1) had extended seed longevity and shallower thermo-dormancy. Compared to the wild type, metabolite profiling of dry Atfahd1a-1 seeds showed that the concentrations of several amino acids, some reducing monosaccharides, and δ-tocopherol dropped, whereas the concentrations of dehydroascorbate, its catabolic intermediate threonic acid, and ascorbate accumulated. Furthermore, the redox state of the glutathione disulphide/glutathione couple shifted towards a more reducing state in dry mature Atfahd1a-1 seeds, suggesting that AtFAHD1a affects antioxidant redox poise during seed development. In summary, AtFAHD1a appears to be involved in seed redox regulation and to affect seed quality traits such as seed thermo-dormancy and longevity. Keywords: Antioxidants, Ascorbate, FAH superfamily, Fumarylacetoacetate hydrolase, Glutathione, Mitochondria, Seed ageing, Seed development, Seed dormancy, Seed longevity Bioblast editor: Reiswig R O2k-Network Lab: AT Innsbruck Jansen-Duerr P


Labels: MiParea: Respiration, Genetic knockout;overexpression  Pathology: Aging;senescence 

Organism: Plants 

Preparation: Intact organ 


Coupling state: ROUTINE  Pathway: CIV  HRR: Oxygraph-2k 

2021-07