Gnaiger 1994 BTK

From Bioblast
Publications in the MiPMap
Gnaiger E, Gellerich FN, Wyss M (1994) What is controlling life? 50 years after Erwin Schrödinger's What is life? Modern trends in BiothermoKinetics 3, Innsbruck Univ Press:336 pp. ISBN 10: 3901249176, ISBN 13: 9783901249174

Gnaiger Erich, Gellerich FN, Wyss M (1994) Innsbruck Univ Press



Erwin Schrödinger's 'What is Life?' is one of the most influential books of the last century in the biological sciences. It continues to provoke discussions and stimulate interdisciplinary developments in biophysics. Starting with reflections on Schrödinger's famous account of negative entropy in living systems, an international group of scientists presents the latest advancements on the dynamics and control of metabolic processes. This volume of Modern Trends in BioThermoKinetics develops for the first time the explicit link between thermodynamics, metabolic control, bioenergetics and clinical applications.

• O2k-Network Lab: AT Innsbruck Gnaiger E, DE Magdeburg Gellerich FN

Labels: MiParea: Respiration, mt-Awareness 

Coupling state: OXPHOS 

HRR: Oxygraph-2k, Theory 

Contents »Bioblast link«


  1. Margreiter R (Innsbruck) p 13. »Bioblast link«
  2. Gnaiger E, Gellerich FN (Innsbruck) Trends in bioenergetics. An introduction. p 14. »Bioblast link«
  3. Oberkofler G, Goller P (Innsbruck) "German Ideology" as seen by Erwin Schrödinger in letters to Hans Thirring. From their correspondence (1946-1949). p 19. »Bioblast link«


Part 1. What is negative entropy? From world views to bioenergetics

  1. Götschl J (Graz) Erwin Schrödinger's world view. The role of physics and biology in his philosophical system. p 23. »Bioblast link«
  2. Wagensberg J (Barcelona) Science, art and revelation. p 32. »Bioblast link«
  3. Azzone GF (Padova) Negentropy and historical arrow of time - thermodynamical and informational aspects of the Darwinian revolution. p 38. »Bioblast link«
  4. Lantos CP (Buenos Aires) Negative entropy: another approach. p 45. »Bioblast link«
  5. Welch GR (New Orleans) Schrödinger's What is Life?: the biophysical legacy 50 years later. p 48. »Bioblast link«
  6. Ho MW (London) What is Schrödinger's negentropy? p 50. »Bioblast link«
  7. Gnaiger E (Innsbruck) Negative entropy for living systems. Controversy between Nobel laureates Schrödinger, Pauling and Perutz. p 62. »Bioblast link«
  8. Kushmerick MJ (Seattle) A paradigm for energy balance in muscle function. p 71. »Bioblast link«
  9. Lloyd D (Cardiff) A controlled chaotic attractor controls life. p 77. »Bioblast link«
  10. Azzone GF (Padova) Disease as instability, error and entropy. p 81. »Bioblast link«


Part 2. Control and regulation: theory, models, and experi­ment

  1. Kholodenko BN, Westerhoff HV, Cascante M (Moscow, Amsterdam, Barcelona) The macro- and microworld of control theory. p 88.
  2. Hofmeyr JHS, Cornish-Bowden A (Stellenbosch, Marseille) How should we quantify metabolic regulation? p 91.
  3. Westerhoff HV, Jensen PR, Rohwer JM, Kholo­denko BN (Amsterdam, Lyngby, Moscow) Control and regulation: the best of both worlds. p 95.
  4. Heinrich R, Klipp E, Stephani A, Wilhelm T (Berlin) Evolutionary optimization of enzymes on the basis of kinetic and thermodynamic principles. p 99.
  5. Schuster S, Hilgetag C, Fell DA (Berlin, Oxford) Detecting elementary modes of functioning in metabolic networks. p 103.


Part 3. Coupling and mechanisms in the regulation of electron, ATP and ion fluxes

  1. Luvisetto S, Canton M, Schmehl I, Azzone GF (Padova) Leaks and slips during mitochondrial electron trans­fer. p 122.
  2. Brand MD, Chien LF, Diolez PH (Cambridge, Bordeaux) Experimental discrimination between proton leak and redox slip during mitochondrial electron transport. p 125.
  3. Canton M, Luvisetto S, Schmehl I, Azzone GF (Padova) Permeability of the inner mitochondrial membrane and respiration. p 129.
  4. Schmehl I, Canton M, Luvisetto S, Azzone GF (Padova) The uncoupling mechanism of the anesthetic bupivacaine. p 133.
  5. Leverve X, Fontaine E, Espié P, Keriel C, Guérin B, Rigoulet M (Grenoble, Bordeaux) Influence of the mechanism of change in ATP synthase-ATPase. Stoichiometry on the control of oxidative phosphorylation in situ. p 137.
  6. Ouhabi R, Boue-Grabot M, Mazat JP (Bordeaux) ATP synthesis in permeabilized cells: assessment of the ATP/O ratios in situ. p 141.
  7. Fitton V, Ouhabi R, Guérin B, Rigoulet M (Bordeaux) Permeabilized spheroplasts as a tool for studying the mechanistic stoichiometry of yeast oxidative phosphory­la­tion. p 145.
  8. Manon S, Roucou X, Guérin M (Bordeaux) Investigations upon the effect of monovalent cations on oxidative phosphorylation in yeast mitochondria: p 150.
  9. Sparagna G, Gunter KK, Sheu SS, Gunter TE (Rochester) Can mitochondria sequester calcium from physiological calcium pulses? p 154.
  10. Ichas F, Jouaville LS, Sidash SS, Mazat JP, Holmuhamedov EL (Bordeaux, Pushchino, Kalamazoo) Mitochondrial calcium spiking: the physiological face of permeability transition. p 159.
  11. Dauncey MJ, Clausen T, Harrison AP (Cambridge, Århus) Developmental regulation of Na+,K+- and Ca2+-ATPases in muscle: p 163.
  12. Siegenbeek van Heukelom J et al (Amsterdam, Moscow) What is controlling the cell membrane potential? p 169.


Part 4. The microenvironment of mitochondria and cells: diffusion gradients, shuttles and signals

  1. Saks VA, Vasilyeva EV, Khuchua ZA, Belikova YO, Aliev MK, Kesvatera T, Tiivel T, Anflous K, Ventura-Clapier R, Keriel C, Fontaine E, Leverve X (Tallinn, Moscow, Paris, Grenoble) ADP diffusion gradients and metabolic oscillatory signals to mitochondria in heart and liver cell. p 178.
  2. Gellerich FN, Laterveer FD, Gnaiger E, Nicolay K (Utrecht, Innsbruck) Effect of macromolecules on ADP-transport into mitochondria. p 181*. »Bioblast link«
  3. Laterveer FD, Gellerich FN, Gnaiger E, Nicolay K (Utrecht, Innsbruck) Macromolecules increase the channeling of ADP from mitochondrially associated hexokinase to the mitochondrial matrix. p 186*. »Bioblast link«
  4. Méndez G, Gnaiger E (Innsbruck, Buenos Aires) How does oxygen pressure control oxygen flux in isolated mitochondria? A methodological approach by high-resolution respirometry and digital data analysis: p 191*. »Bioblast link«
  5. Soboll S, Conrad A, Gruwel M (Düsseldorf) Determination of creatine kinase fluxes with purified creatine kinase and isolated mitochondria using 31P nuclear magnetic resonance. p 195.
  6. Hoerter JA, Gillet B, Mateo P, Lechene P, Beloeil JC (Chatenay Malabry, Gifs/Yvette) Oscillations of intracellular pH or high energy phosphate content cannot be detected by gated NMR during the cardiac cycle of an isovolumic perfused rat heart. p 199.


Part 5. The impact of bioenergetics on cellular, physiological and biotechnological processes

  1. Gnaiger E, Wyss M (Innsbruck) Chemical forces in the cell: calculations for the ATP system. p 207. »Bioblast link«
  2. Jeneson JAL, Jubrias SA, Kushmerick MJ (Utrecht, Seattle) The flow-force relation of oxidative phosphorylation in human forearm muscle is sigmoidal. p 213.
  3. Mejsnar J, Marsík F (Praha) Muscular contraction, dissipation and Schrödinger's negative entropy. p 215.
  4. Jensen PR, Rohwer JM, Michelsen O, Westerhoff HV (Lyngby, Amsterdam) Modelling of oxidative phosphorylation in E. coli. p 218.
  5. Fishov I (Beer-Sheva) Do oscillations control the bacterial cell cycle? p 221
  6. Kemp RB, Hoare S, Schmalfeldt M, Bridge CMC, Evans PM, Gnaiger E (Aberystwyth, Innsbruck) A thermochemical study of the production of lactate by glutaminolysis and glycolysis in mouse macrophage hybridoma cells. p 226. »Bioblast link«
  7. Larsson C, Nilsson A, Gustafsson L (Göteborg) Catabolic capacity of carbon- or nitrogen-starved cultures of Saccharomyces cerevisiae. p 232.
  8. Krumschnabel G, Wieser W (Innsbruck) Energy allocation and material flux in fish hepatocytes under stress. p 238.
  9. Marx A, de Graaf AA, Wiechert W, Eggeling L, Sahm H (Jülich) Carbon flux in lysine producing strain Corynebacterium glutamicum MH20-22B: analysis by 13C-NMR of amino acids and metabolite balancing. p 240.
  10. Zentgraf B (Furtwangen) Remarks on biocalorimetry and biothermodynamics aimed at characterization of the physiological state. p 244.


Part 6. The impact of bioenergetics on clinical and pathologi­cal problems

  1. Petronilli V, Nicolli A, Costantini P, Colonna R, Bernardi P (Padova) The permeability transition pore. Pathophysiology of a cyclosporin A-sensitive mitochondrial channel. p 259.
  2. Gellerich FN, Steinlechner R, Wyss M, Eberl T, Müller LC, Skladal D, Sperl W, Dapunt O, Margreiter R, Gnaiger E (Innsbruck) Mitochondrial function in the atrial appendage of the human heart. Characterization by high-resolution respirometry. p 263*. »Bioblast link«
  3. Skladal D, Sperl W, Schranzhofer R, Krismer M, Gnaiger E, Margreiter R, Gellerich FN (Innsbruck) Preservation of mitochondrial functions in human skeletal muscle during storage in high energy preservation solution (HEPS). p 268*. »Bioblast link«
  4. Mazat JP, Letellier T, Malgat M, Jouaville S, Morkuniene R (Bordeaux, Kaunas) Application of control analysis to the study of metabolic diseases in mitochondria. Metabolic expression of mitochondrial DNA mutations. p 272.
  5. Kuznetsov AV, Clark JF, Winkler K, Kunz WS (Magdeburg, Oxford) Change in flux control coefficient of cytochrome c oxidase in copper deficient mottled brindled mice. p 275*. »Bioblast link«
  6. Oexle K, Oberle J, Hübner C. Insulin-dependent diabetes mellitus in MELAS-mitochondriopathy: discussion of possible causal relations. p 278.
  7. Jeneson JAL, Westerhoff HV (Utrecht, Amsterdam) Simulation of the flow-force relation of oxidative phosphor­ylation in Complex I deficiency. p 280.
  8. Steinlechner R, Eberl T, Margreiter R, Gnaiger E (Innsbruck) Oxygen dependence of cellular respiration in endothelial cells: a sensitive toxicological test. p 283*. »Bioblast link«
  9. Eberl T, Steinlechner R, Salvenmoser W, Abendstein B, Rhomberg M, Schröcksnadel H, Schmid T, Gnaiger E, Margreiter R (Innsbruck) Endothelial cell cultures as a model for organ preservation studies. p 288*. »Bioblast link«
  10. Knoblechner A, Steinlechner R, Schirmer M, Gellerich FN, Margreiter R, Konwalinka G, Gnaiger E (Innsbruck) The effect of 2-chlorodeoxyadenosine on endogenous respiration of a human lymphoma cell line (U-937). p 294*. »Bioblast link«

Part 7. Computer software for bioenergetics and metabolic control analysis

  1. Mendes P, Kell DB (Aberystwyth) Simultaneous estimates of single-enzyme kinetic parameters for multi-enzymatic systems using artificial neural networks. p 302.
  2. Wiechert W (Jülich) Design of a software framework for flux determination by 13C NMR isotope labelling experiments. p 305.


  • Index

Subject index: p 319

Author index: p 325

Addresses of participants: p 329

Cited by

Gnaiger 2024 Ambiguity crisis.jpg
Gnaiger E (2024) Addressing the ambiguity crisis in bioenergetics and thermodynamics. MitoFit Preprints 2024.3.


Gnaiger 2024 MitoFit 

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