Goedecke 2017 JMIR Res Protoc

From Bioblast
Jump to: navigation, search
Publications in the MiPMap
Goedecke JH, Mendham AE, Clamp L, Nono Nankam PA, Fortuin-de Smidt MC, Phiri L, Micklesfield LK, Keswell D, Woudberg NJ, Lecour S, Alhamud A, Kaba M, Lutomia FM, van Jaarsveld PJ, de Villiers A, Kahn SE, Chorell E, Hauksson J, Olsson T (2017) An exercise intervention to unravel the mechanisms underlying insulin resistance in a cohort of black South African women: Protocol for a randomized controlled trial. JMIR Res Protoc 03/10/2017:9098.

» Open Access

Goedecke JH, Mendham AE, Clamp L, Nono Nankam PA, Fortuin-de Smidt MC, Phiri L, Micklesfield LK, Keswell D, Woudberg NJ, Lecour S, Alhamud A, Kaba M, Lutomia FM, van Jaarsveld PJ, de Villiers A, Kahn SE, Chorell E, Hauksson J, Olsson T (2017) JMIR Res Protoc

Abstract: The pathogenesis of type 2 diabetes (T2D) in black African women is complex and differs to that of their white counterparts. However, earlier studies have been cross-sectional and provide little insight into the causal pathways. Exercise training is consistently used as a model to examine the mechanisms underlying insulin resistance and risk for T2D.

To examine the mechanisms underlying the changes in insulin sensitivity and secretion in response to a 12-week exercise intervention in obese black South African (SA) women.

Forty-five obese (BMI 30-40 kg/m2) black SA women were randomized into a control (n=22) or experimental (exercise; n=23) group. The exercise group completed 12 weeks of supervised combined aerobic and resistance training (40-60 min, 4 days/week), while the control group maintained their typical physical activity patterns, and both groups were requested not to change their dietary patterns. Prior to and following the 12-week intervention period, insulin sensitivity and secretion (frequently sampled intravenous glucose tolerance test), and its primary and secondary determinants, were measured. Dietary intake, sleep quality and quantity, physical activity and sedentary behaviors were measured every four weeks.

The final sample included 20 exercise and 15 control participants. Baseline socio-demographics, cardiorespiratory fitness, anthropometry, cardio-metabolic risk factors, physical activity and diet did not differ between the groups (p>0.05).

We describe a research protocol for an exercise intervention to understand the mechanisms underlying insulin sensitivity and secretion in obese black SA women. We aim to identify causal pathways underlying the high prevalence of insulin resistance and risk for T2D in black SA women, targeting specific areas for therapeutic intervention.

Keywords: Amplex Red in muscle fibers Bioblast editor: Kandolf G


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style  Pathology: Diabetes, Obesity 

Organism: Human  Tissue;cell: Skeletal muscle, Fat  Preparation: Permeabilized tissue 


Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, S, CIV, NS, ROX  HRR: Oxygraph-2k, O2k-Fluorometer 

2018-01, Amplex UltraRed, BMI, VO2max