Granata 2022 Abstract Bioblast
|Granata Cesare (2022) Exercise training-induced enhancement of electron flow to the OXPHOS system is more important than increasing the OXPHOS machinery content to improve ATP generation in human skeletal muscle. Bioblast 2022: BEC Inaugural Conference.|
Granata Cesare (2022)
Event: Bioblast 2022
Mitochondrial health is implicated in multiple diseases and ageing, and is therefore an important determinant of an individual’s quality of life . Exercise training is an accessible and inexpensive therapeutic intervention that is extensively used to prevent, treat, and manage several lifestyle diseases , by enhancing mitochondrial biogenesis and improving mitochondrial bioenergetics. However, the intricacy of exercise training-induced mitochondrial adaptations remains, for the most part, unknown.
By utilizing a multi-omics approach integrated with classic biological mitochondrial techniques we performed an in-depth investigation of the effects of three different and sequential volumes of high-intensity interval training on the mitochondrial transcriptome, proteome, and lipidome in human skeletal muscle (n=10) .
We first confirmed that changes in mitochondrial respiration, content, and enzymatic activity, as well as supercomplex formation and the content of selected subunits of the OXPHOS system mirrored, for the most part, the changes in training volume, and that these changes were driven by the overall increase in mitochondrial content, as previously demonstrated . Subsequently, by combining the power of 3 omics techniques with biochemical and in silico normalization, we removed the bias arising from the training-induced increase in mitochondrial content to unearth an intricate and previously undemonstrated network of differentially prioritized mitochondrial adaptations. We show that changes in hundreds of transcripts, proteins, and lipids are not stoichiometrically linked to the overall increase in mitochondrial content. Our findings indicate that enhancing electron flow to the OXPHOS system is more important to improve ATP generation than increasing the abundance of the OXPHOS machinery, and do not support the hypothesis that training-induced supercomplex reorganization enhances mitochondrial bioenergetics .
Our study provides an analytical approach allowing unbiased and in-depth investigation of training-induced mitochondrial adaptations that challenges our current understanding and calls for careful reinterpretation of previous findings.
- Institute for Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research, Heinrich Heine Univ, 40225 Düsseldorf, Germany
- German Center for Diabetes Research, Partner Düsseldorf, München-Neuherberg, Germany. –email@example.com
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Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Organism: Human Tissue;cell: Skeletal muscle