Granata 2022 Abstract Bioblast
|3.3. «5 min» https://doi.org/10.26124/bec:2022-0001|
Granata Cesare (2022)
Event: Bioblast 2022
Mitochondrial health is implicated in multiple diseases and ageing, and is therefore an important determinant of an individual’s quality of life . Exercise training is an accessible and inexpensive therapeutic intervention that is extensively used to prevent, treat, and manage several lifestyle diseases , by enhancing mitochondrial biogenesis and improving mitochondrial bioenergetics. However, the intricacy of exercise training-induced mitochondrial adaptations remains, for the most part, unknown.
By utilizing a multi-omics approach integrated with classic biological mitochondrial techniques, an in-depth investigation of the effects of three different and sequential volumes of high-intensity interval training on the mitochondrial transcriptome, proteome, and lipidome was performed in human skeletal muscle (N=10) .
Changes in mitochondrial respiration, enzymatic activity, supercomplex formation, and the content of selected subunits of the OXPHOS system mirrored, for the most part, changes in training volume, and were driven by the overall increase in mitochondrial content, as previously demonstrated . Subsequently, by combining the power of 3 omics techniques with biochemical and in silico normalization, the bias arising from the training-induced increase in mitochondrial content was removed to unearth an intricate and previously undemonstrated network of differentially prioritized mitochondrial adaptations. These findings indicate that enhancing electron flow in the OXPHOS system is more important to improve ATP generation than increasing the abundance of the OXPHOS machinery, and do not support the hypothesis that training-induced supercomplex reorganization enhances mitochondrial bioenergetics .
This study provides an analytical approach allowing unbiased and in-depth investigation of training-induced mitochondrial adaptations that challenges our current understanding and calls for careful reinterpretation of previous findings.
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- Granata C, Caruana NJ, Botella J, Jamnick NA, Huynh K, Kuang J, Janssen HA, Reljic B, Mellett NA, Laskowski A, Stait TL, Frazier AE, Coughlan MT, Meikle PJ, Thorburn DR, Stroud DA, Bishop DJ (2021) High-intensity training induces non-stoichiometric changes in the mitochondrial proteome of human skeletal muscle without reorganisation of respiratory chain content. http://hdl.handle.net/11343/296256
- Granata C, Oliveira RS, Little JP, Renner K, Bishop DJ (2016) Mitochondrial adaptations to high-volume exercise training are rapidly reversed after a reduction in training volume in human skeletal muscle. https://doi.org/10.1096/fj.201500100R
- Institute for Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research, Heinrich Heine Univ, 40225 Düsseldorf, Germany
- German Center for Diabetes Research, Partner Düsseldorf, München-Neuherberg, Germany – email@example.com
Present version: v2 Previous version and comments by reviewer's: Talk:Granata 2022 Abstract Bioblast
List of abbreviations, terms and definitions - MitoPedia
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Organism: Human Tissue;cell: Skeletal muscle
HRR: Oxygraph-2k Event: A3