Hecker 2015 Methods Mol Biol
|Hecker M, Sommer N, Mayer K (2015) Assessment of short- and medium-chain fatty acids on mitochondrial function in severe Inflammation. Methods Mol Biol 1265:389-96.|
Abstract: Mitochondrial dysfunction is regarded as one key factor involved in the pathogenesis of septic disorders, leading to a decline in energy supply. The infl uence of short- and medium-chain fatty acids (SCFA/MCFA) on mitochondrial respiration under infl ammatory conditions has thus far not been investigated. In the following protocol, we describe the assessment of mitochondrial respiration using high-resolution respirometry under infl ammatory and baseline conditions. For this approach, human endothelial cells and monocytes were pretreated with TNF-α to mimic infl ammation followed by incubation with SCFA/MCFA and then subjected to high-resolution respirometry. Mitochondrial DNA content was assessed by PCR.
• Keywords: Mitochondria, Respiration, Oxygen consumption, SCFA, MCFA, TNF-α, HUVEC
• O2k-Network Lab: DE Giessen Weissmann N
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, mtDNA;mt-genetics Pathology: Sepsis
Organism: Human Tissue;cell: Endothelial;epithelial;mesothelial cell Preparation: Intact cells
Regulation: Coupling efficiency;uncoupling, Uncoupler, Fatty acid Coupling state: LEAK, ROUTINE, ET Pathway: ROX HRR: Oxygraph-2k