Hedges IBEC2012

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Hedges CP, Hickey AJ, McMahon CD, Smith HK (2012) A fiber-type-specific role for myostatin in regulating mitochondrial function. IBEC2012.

Link:

Hedges C, Hickey AJ, McMahon CD, Smith HK (2012)

Event: 15th International Biochemistry of Exercise Conference

Aim: This study aimed to compare mitochondrial oxygen consumption in C57BL/6J wild-type and myostatin-deficient mouse soleus and white gastrocnemius muscles.

Methods: Muscles were obtained from 4 month-old male mice. Mass-specific oxygen consumption (pmol.O2/mg/sec) was measured in permeabilised muscle, using high-resolution respirometry (Oroboros Oxygraph-2k). Maximal activities of citrate synthase and lactate dehydrogenase were determined by spectrophotometry.

Results: Myostatin-deficient soleus (n=6) consumed 20% more oxygen per mg than wild-type soleus (n=6) during oxidative phosphorylation. This was accompanied by greater citrate synthase and lactate dehydrogenase enzyme activity in myostatin-deficient soleus (29% and 80% respectively). Myostatin-deficient gastrocnemius (n=7) showed 26% lower oxygen consumption during uncoupled respiration, and 34% lower oxygen consumption when oxidising glycerol-3-phosphate compared to wild-type gastrocnemius (n=8). Citrate synthase activity was not significantly different and lactate dehydrogenase activity 26% greater in myostatin-deficient gastrocnemius compared to wild-type gastrocnemius.

Conclusion: These data suggest that myostatin-deficiency exerts fiber-type specific effects on skeletal muscle mitochondrial function.


O2k-Network Lab: NZ Auckland Hickey AJ, AU Melbourne Stepto NK


Labels: MiParea: Respiration, Genetic knockout;overexpression, Comparative MiP;environmental MiP 


Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue  Enzyme: TCA cycle and matrix dehydrogenases  Regulation: Substrate  Coupling state: OXPHOS 

HRR: Oxygraph-2k, O2k-Spectrophotometer