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Heichler 2022 BEC

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Bioenergetics Communications        
Gnaiger 2020 BEC MitoPathways
       
Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1.
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Bioenergetics Communications
Publications in the MiPMap
Heichler C, Nagy M, Wolf P (2022) Evaluation of hepatotoxic effects of acetaminophen on HepG2 cells by parallel real-time monitoring in a multi-sensor analysis platform for automated cell-based assays. https://doi.org/10.26124/bec:2022-0006

» Bioenerg Commun 2022.6. Open Access pdf
published online 2022-07-06 »Watch the presentation«

Heichler Christina, Nagy Marton, Wolf Peter (BEC 2022.06) Bioenerg Commun

Abstract: BEC.png https://doi.org/10.26124/bec:2022-0006 The CYRIS® analysis platform is a multi-sensory approach to extract a large amount of information from a single cell-based assay automatically and in real-time. To demonstrate its capabilities, we performed an in vitro hepatotoxicity assay with acetaminophen and HepG2, with simultaneous monitoring of the key parameters oxygen consumption rate (OCR), extracellular acidification rate (ECAR), impedance, and microscopic imaging. After 12 hours prior treatment measurement, different concentrations of acetaminophen were tested over 24 hours, followed by 12 hours washout. The metabolic results showed a strong time- and dose-dependent change of OCR and ECAR through acetaminophen. Morphologic changes monitored by impedance and microscopic imaging underpin these metabolic effects. The washout of acetaminophen results in cellular regeneration in all parameters up to a concentration of 10 mM. The continuous measurement of OCR, ECAR, impedance, and microscopic imaging enables multiparametric monitoring of cellular metabolic responses due to acetaminophen in a single assay and provides an overall picture of its hepatotoxic effects.

Keywords: label-free cell-based assays, oxygen consumption, extracellular acidification, cellular impedance, imaging, automation, hepatotoxicity, acetaminophen Bioblast editor: Tindle-Solomon L

ORCID: ORCID.png Heichler Christina ORCID.png Nagy Márton

Preprint

» Heichler 2022 MitoFit


Labels: MiParea: Respiration, Instruments;methods, Pharmacology;toxicology 

Stress:Cell death  Organism: Human  Tissue;cell: Liver, Other cell lines  Preparation: Intact cells 


Coupling state: ROUTINE 


BEC