High signal at zero oxygen

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High signal at zero oxygen


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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

A high signal at zero oxygen may be observed during zero calibration. The following instructions show how to further localize and possibly solve this technical problem.

Abbreviation: R0

Reference: Zero calibration, OroboPOS-Connector

High zero current test

1. Unscrew the POS from the POS-Connector and unplug the POS-Connector from the O2k-main unit.
2. Switch on the O2k and start DatLab.
3. Record the signal for 10 min.
4. Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector".
5. Record the signal for 10 min.
6. Remove the membrane from the POS and wash the cathode and anode with distilled water.
7. Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
8. Record the signal for 10 min.
9. Send us the file.


References:
[1] Cleaning the electrical connections
[2] MiPNet19.18B POS-service
[3] MiPNet06.03 POS-calibration-SOP
[4] MiPNet14.06 Instrumental O2 background
Example: First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O2 raw signal [V] was recorded for 10 min. The marks were set to the O2 raw signal [V] to evaluate the zero current.
High zero current good example.png
High zero current test bad exmaple.png
Figure 1. High zero current test. Upper panel: Zero current is in the range of ±0.00099 V. Lower panel: Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem with both the OroboPOS-Connector and the OroboPOS.

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